This is a famously fabricated crystal structure - the absence of
contact was one of the clues, see
http://www.nature.com/nature/journal/v448/n7154/full/nature06102.html
Cheers, Matt.
On 2015-02-06 11:58, Kerff Fred wrote:
Hello,
Looking at structure 2HR0 ("The structure of complement C3b provides
insights into complement activation and regulation. »,Abdul Ajees,
A.,
Gunasekaran, K., Volanakis, J.E., Narayana, S.V., Kotwal, G.J.,
Krishna Murthy, H.M.; (2006) Nature 444: 221-225), I noticed the
absence of contacts between layers in the crystal. Is it something
that has already been observed in other crystals?
Best regards,
Fred
-----
Frédéric Kerff
Chercheur qualifié F.R.S.-FNRS
Cristallographie des protéines
Centre d'Ingénierie des Protéines
Université de Liège
17, Allée du 6 Août - Bat B5a
4000 Liège (Belgium)
Tel.: +32 (0)4 3663620
Fax: +32 (0)4 3663772
Le 6 févr. 2015 à 10:12, Tim Gruene <t...@shelx.uni-ac.gwdg.de> a
écrit :
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Hash: SHA1
Dear Smith,
The sca file most likely does not contain flags. pointless can read
the sca file, standardise it to ccp4 standards and freerflag marks
random reflections. You should use the maximum of 500 unique
reflections or 5% of the unique reflections, whichever is larger.
Best,
Tim
On 02/06/2015 09:49 AM, Smith Lee wrote:
Dear All, I have a sca file. Will you please tell me by which
software or how I can know whether the sca file contains R-free
tags? If not, by which software or how I can add the R-free tags?
And how much of the reflections I add the R-free tags? I am looking
forward to getting your reply. Smith
- --
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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--
Matthew Bowler
Synchrotron Diffraction Group
European Molecular Biology Laboratory
71, avenue des Martyrs
CS 90181
F-38042 GRENOBLE Cedex 9
FRANCE
France
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