Sebastiano,
I have had really nice success by mixing initial crystallization hits back in to sparse matrix screen conditions. My personal favourite is using the Wizard screens. http://www.rigakureagents.com/p-1-wizard-classic-crystallization-screen-series.aspx I found 75% of the original condition mixed with 25% of a sparse matrix screen results in many drops containing crystals with different habits. This allowed me to get around anisotropy and to improve the resolution from poorly diffracting crystals. A primary reference for this is found here: Acta Crystallogr D Biol Crystallogr.<https://www.ncbi.nlm.nih.gov/pubmed/15858279> 2005 May;61(Pt 5):646-50. Epub 2005 Apr 20. Crystallization of foot-and-mouth disease virus 3C protease: surface mutagenesis and a novel crystal-optimization strategy. Birtley JR1, Curry S. Good luck with your experiments. James ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Andreas Forster [docandr...@gmail.com] Sent: 08 May 2017 09:57 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] looking for paper describing optimisation of crystals using a screen kit as additive Hi Sebastian, you're thinking about local sparse matrix screening. I've done this at a 90:10 ratio. Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., and Kwong, P.D. Enhancing protein crystallization through precipitant synergy. Structure. 2003; 11: 1061–1070 All best. Andreas On Mon, May 8, 2017 at 3:18 PM, Sebastiano Pasqualato <sebastiano.pasqual...@gmail.com<mailto:sebastiano.pasqual...@gmail.com>> wrote: Dear all, I recall a paper (or was is a talk at a conference?) describing the optimisation of initial hits of crystallisation by using a standard screen kit as additive. Something like setting the tray using the initial crystallisation hit condition in the reservoir and mixing 75% of the hit condition with 25% of a commercial sparse matrix screen kit with the protein in the drop. I can’t find the reference, can anybody help me? Thanks a lot, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167<tel:+39%2002%209437%205167> fax +39 02 9437 5990<tel:+39%2002%209437%205990> web http://is.gd/IEOXtalUnit
