Dear all, as usual the bulletin board nails it. The paper I was vaguely remembering was indeed the following:
Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):646-50 Crystallization of foot-and-mouth disease virus 3C protease: surface mutagenesis and a novel crystal-optimization strategy. Birtley JR, Curry S. But of course, the bulletin board goes even further, providing other interesting hints and food for thoughts: Structure. 2003 Sep;11(9):1061-70. Enhancing protein crystallization through precipitant synergy. Majeed S, Ofek G, Belachew A, Huang CC, Zhou T, Kwong PD. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70(Pt 9):1117-26 Microseed matrix screening for optimization in protein crystallization: what have we learned? D'Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M Structure. 2017 Jan 3;25(1):107-120 Binding of Myomesin to Obscurin-Like-1 at the Muscle M-Band Provides a Strategy for Isoform-Specific Mechanical Protection. Pernigo S, Fukuzawa A, Beedle AE, Holt M, Round A, Pandini A, Garcia-Manyes S, Gautel M, Steiner RA. http://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf <http://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf> Thanks everyone for the feedback and help! May the crystals grow happily and diffract nicely, ciao, Sebastiano > On 8 May 2017, at 15:18, Sebastiano Pasqualato > <sebastiano.pasqual...@gmail.com> wrote: > > > Dear all, > I recall a paper (or was is a talk at a conference?) describing the > optimisation of initial hits of crystallisation by using a standard screen > kit as additive. > Something like setting the tray using the initial crystallisation hit > condition in the reservoir and mixing 75% of the hit condition with 25% of a > commercial sparse matrix screen kit with the protein in the drop. > I can’t find the reference, can anybody help me? > Thanks a lot, > ciao, > Sebastiano > > > -- > Sebastiano Pasqualato, PhD > Crystallography Unit > Department of Experimental Oncology > European Institute of Oncology > IFOM-IEO Campus > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5167 > fax +39 02 9437 5990 > web http://is.gd/IEOXtalUnit <http://is.gd/IEOXtalUnit>
