That worked - Thanks so much! On Thu, Sep 13, 2012 at 4:37 PM, Drummond, Iain A. <idrumm...@partners.org> wrote: > I ran into (I think) similar problems running primer3 with Emboss eprimer3. > > It turned out that the most recent version of primer3 (2.x.x) will not run > with the current Emboss eprimer3; you have to downgrade primer3 to 1.1.4 to > get it to work. > > The Emboss folks know this I think. > > -Iain > > > ------- > Iain Drummond, Ph.D. > Associate Professor > Nephrology Division, Massachusetts General Hospital, > Department of Genetics, Harvard Medical School and > Program in Developmental and Regenerative Biology, Harvard Medical School > > Address for mailing: > > Nephrology Division, MGH > 149 13th Street, Rm 149-8000 > Charlestown MA 02129 > > 617 726 5647 (office) > 617 724 9693 (lab) > 617 726 5669 (fax) > > idrum...@receptor.mgh.harvard.edu > idrumm...@partners.org > > HTTP://danio.mgh.harvard.edu <°){{{{-< > > > > On 9/13/12 4:24 PM, "Paul Tanger" <paul.tan...@colostate.edu> wrote: > >> Hi, >> I have a local install of emboss and I'm trying to get eprimer3 to >> work, but I'm getting this error: >> >> "Error: thermodynamic approach chosen, but path to thermodynamic >> parameters not specified" >> >> primer3 is installed, but not in the default location (which I think >> is /opt/primer3_config ?) . >> How do I specify where primer3 is installed? Or is the cause of this >> error something else? >> >> googled for an answer for a while, but couldn't find one. >> >> Thanks! >> _______________________________________________ >> EMBOSS mailing list >> EMBOSS@lists.open-bio.org >> http://lists.open-bio.org/mailman/listinfo/emboss >> > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine > at > http://www.partners.org/complianceline . If the e-mail was sent to you in > error > but does not contain patient information, please contact the sender and > properly > dispose of the e-mail. >
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