Hi Paul,

which version of primer3 do you use? What operating system? How did you install primer3 and EMBOSS?

If it is primer3 version 2.* I think you should use eprimer32 wrapper instead and it expects primer3_core to be called primer32_core. To make primer3 aware of another location of the primer3_config directory: it seems that two files need to be modified before compiling: thal_main.c and primer3_boulder_main.c (in the subfolder src).

Hope this helps.

regards,

Petar


Quoting Paul Tanger <paul.tan...@colostate.edu>:

Hi,
I have a local install of emboss and I'm trying to get eprimer3 to
work, but I'm getting this error:

"Error: thermodynamic approach chosen, but path to thermodynamic
parameters not specified"

primer3 is installed, but not in the default location (which I think
is /opt/primer3_config ?) .
How do I specify where primer3 is installed?  Or is the cause of this
error something else?

googled for an answer for a while, but couldn't find one.

Thanks!
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