Thanks, Liam! It worked perfectly.

-Rafa

*--*
*Rafael S. Marcondes, Ph.D.*
*https://www.rafaelmarcondes.com/ <https://www.rafaelmarcondes.com/>*
Faculty Fellow in EEB
Department of BioSciences
Rice University
Houston TX 77005

Pronouns: he/him


*"Eu quase que nada não sei. Mas desconfio de muita coisa"*
*"I almost don't know nothing. But I suspect many things"*
  -João Guimarães Rosa, Brazilian novelist
(Portuguese original and free English translation by me)


On Tue, Apr 18, 2023 at 1:35 PM Liam J. Revell <liam.rev...@umb.edu> wrote:

> Dear Rafa & list.
>
> Here is a solution:
> http://blog.phytools.org/2023/04/coloring-edges-of-plotted-tree-by.html.
>
> I applied it to marginal ancestral states obtained using the new
> *phytools* method *ancr*, but the same principle could be used with a
> summary of stochastic maps. I will make a subsequent follow-up post
> covering that.
>
> Let us know if this works!
>
> All the best, Liam
> Liam J. Revell
> Professor of Biology, University of Massachusetts Boston
> Web: http://faculty.umb.edu/liam.revell/
> Book: Phylogenetic Comparative Methods in R
> <https://press.princeton.edu/books/phylogenetic-comparative-methods-in-r>
> (*Princeton University Press*, 2022)
>
>
> On 4/18/2023 1:21 PM, Liam J. Revell wrote:
>
> Dear Rafa.
>
> Just a quick comment re the comment "I also noticed that results of simmap
> have one extra edge than the tree
> itself...."
>
> From ancestral state reconstruction (marginal reconstruction or a summary
> of stochastic mapping) you'll end up with a matrix containing a number of
> rows equal to the number of internal nodes of the tree, or (in your case)
> the number of tips + the number of internal nodes. In your case, you're
> interested in coloring all the edges of the tree, which will be one fewer
> than the number of nodes + tips. This is because every node (or tip) is
> preceded by one edge: except for the root. Likewise, the order of your
> ancestral state matrix is by node index; whereas the edges of the tree can
> have a variety of different orders.
>
> Let me post a solution to my blog quickly & then I'll share it with this
> group.
>
> All the best, Liam
> Liam J. Revell
> Professor of Biology, University of Massachusetts Boston
> Web: http://faculty.umb.edu/liam.revell/
> Book: Phylogenetic Comparative Methods in R
> <https://press.princeton.edu/books/phylogenetic-comparative-methods-in-r>
> (*Princeton University Press*, 2022)
>
>
> On 4/18/2023 10:59 AM, Rafael S Marcondes wrote:
>
> CAUTION: EXTERNAL SENDER
>
> Hi all,
>
> I would like to use the results of make.simmap to plot the edges of a tree
> by their most likely state. (Because my tree is really large and plotting
> pie charts at the nodes gets really polluted.)
>
> So first I took $ace from the results to describe.simmap and added a column
> for the most likely state, and another column for the color for plotting:
>
>
> head(ace.max.lik)
>
>      CL    EL     M     P SL maxlik            col
> 6621  0 0.000 0.002 0.998  0      P darkgoldenrod3
> 6622  0 0.000 0.001 0.999  0      P darkgoldenrod3
> 6623  0 0.000 0.007 0.993  0      P darkgoldenrod3
> 6624  0 0.000 0.004 0.996  0      P darkgoldenrod3
> 6625  0 0.000 0.001 0.999  0      P darkgoldenrod3
> 6626  0 0.001 0.020 0.979  0      P darkgoldenrod3
>
> And I separately did that for the tips. (Which I also estimated, since they
> had uncertainty in the original data):
>
>                          CL EL M P SL maxlik            col
> Rhea_americana                  0.000        0.000       0.000       1.000
>        0.000      P darkgoldenrod3
> Rhea_pennata                    0.000        0.000       0.008       0.992
>        0.000      P darkgoldenrod3
> Casuarius_casuarius             0.000        0.004       0.074       0.922
>        0.000      P darkgoldenrod3
> Casuarius_bennetti              0.000        0.010       0.132       0.858
>        0.000      P darkgoldenrod3
> Dromaius_novaehollandiae        0.001        0.000       0.045       0.953
>        0.001      P darkgoldenrod3
> Apteryx_owenii                  0.000        0.000       0.995       0.005
>        0.000      M           blue
>
> Then I tried to plot the edges by color using ape, putting the tips in
> front of the internal nodes because that's the typical order of tree$edges:
>
> plot.phylo(x=m.ard[[1]],cex=0.2,edge.width=0.01,type='fan',
>            edge.lty=3,edge.color=c(tip.max.lik$col, ace.max.lik$col))
>
> It plots a beautiful plot, by the colors are mismatched to the edges. I
> also noticed that results of simmap have one extra edge than the tree
> itself:
>
>
> nrow(md.ard$ace)
>
> [1] 13239
>
>
> nrow(m.ard[[1]]$edge)
>
> [1] 13238
>
> I suspect that's that one extra edge that's causing the mismatch, but I
> can't figure out where it's coming from, or how to fix it. Any tips or
> workarounds to accomplish this task, which I might be overcomplicating?
>
> PS: I'm well aware that it's a bad idea to "flatten" the likelihoods of
> different states into a single most likely state, and I absolutely do not
> plan to discard that uncertainty. I just want to plot a simpler tree for
> visualization.
>
> Thanks!
>
> -Rafa
>
> *--*
> *Rafael S. Marcondes, Ph.D.*
> *https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.rafaelmarcondes.com%2F&data=05%7C01%7Cliam.revell%40umb.edu%7C5158c206d6574070f31f08db401dafa8%7Cb97188711ee94425953c1ace1373eb38%7C0%7C0%7C638174268515335805%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=U3PgC3MDqLy06ZNB21FN9r58Q4aBBsqbFyUWJr7AVio%3D&reserved=0
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>  <https://www.rafaelmarcondes.com/>*
> Faculty Fellow in EEB
> Department of BioSciences
> Rice University
> Houston TX 77005
>
> Pronouns: he/him
>
>
> *"Eu quase que nada não sei. Mas desconfio de muita coisa"*
> *"I almost don't know nothing. But I suspect many things"*
>   -João Guimarães Rosa, Brazilian novelist
> (Portuguese original and free English translation by me)
>
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