Re: [base] Réplicates in base 2

2006-12-08 Thread Nicklas Nordborg
Emmanuel Courcelle wrote:
 Hello
 
 We are trying to create an experiment composed with:
 
 -4 slides
 -3 times each reporter on each slide
 
 So, we have up to 12 replicates for each reporter (sometimes less 
 because some flagged spots were filtered out).
 How to make a final bioassay set with 6158 averaged-spots instead of 
 more than 77000 ?


There is no merge plugin available for BASE2. You can try using the 
BASE1 merge plugin which can be found on: 
http://lev.thep.lu.se/trac/baseplugins/wiki/PluginDownload

/Nicklas


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Re: [base] Location of original source files

2006-12-08 Thread Nicklas Nordborg
Charles Girardot wrote:
 Hi Nicklas,
 
 I might have missed something as I am not using BASE2 now and haven't  
 investigated in details. I have a comment on this thread :
 Very often people come up with some logics in naming their files and,  
 in the context of re-exporting them (eg, submission), they like to  
 see file having original names. They might be various other situation  
 where having the same name is good. I think you should keep original  
 names somewhere like in BASE1, it makes things easier.

When you upload files the original filename is kept in the database. 
There is however no information that links a rawbioassay to the file 
that was used for importing the data. It has nothing to do with the file 
names.

/Nicklas


 
 Am I off track?
 
 Cheers
 
 Charles
 
 On Dec 8, 2006, at 9:00 AM, Nicklas Nordborg wrote:
 
 Keith Ching wrote:
 Hi,

 I am trying to write a plugin that will download the original source
 files used to
 populate the RawBioAssay objects.  When you import data into a  
 RawBioAssay,
 is the name of the original source file saved somewhere in the  
 database?

 I am at the point where i can select the RawBioAssays that i want to
 download.  It's
 just I can't find what file was originally used to create it.
 That information is not saved.

 /Nicklas


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 =
 Charles Girardot
 European Molecular Biology Laboratory
 C. Boulin and E. Furlong Groups
 Tel: +49-6221-387-8426
 Email: [EMAIL PROTECTED]
 Meyerhofstrasse 1 - Postfach 10.2209
 69012 Heidelberg, Germany
 =
 
 
 
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[base] Conversion from string to int after plate import

2006-12-08 Thread david vilanova

Hi,
I'm importing the following plate information through the plate 384_wells
import plate.

384_number 384_column 384_row 384_position oligo_id
plate_1 1 A A1 SEQ12345
plate_1 2 A A2 SEQ12346
plate_1 3 A A3 SEQ12347

The importing works fine but the row letters are converted into strings and
+1 is added to the column values.

For example the row value A is converted into 0 and the col value 1 is
converted into 0. Is this the expected behaviour ?
How can i resolve this issus.?

Also note the the testfile option available through the importer does get
the values properly without changing anything.

thanks ,

david
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Re: [base] Conversion from string to int after plate import

2006-12-08 Thread david vilanova

Hi,
After checking the sql structure of the database I realized that the table
PlateMappingDetails contains indeed int values for all fields. I would have
imagined that one could eventually use a letter instead of an integer.
What do you think ?
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Re: [base] Location of original source files

2006-12-08 Thread Keith Ching
There is one place that i found the linkage of the RawBioAssay to the 
original file..
the job parameters..  when you import data into the RawBioAssay it 
creates a job that
details the RawBioAssay and the file path..  however, users can delete 
the jobs and thus

wipe out the connection.. ( which i have done as well )

however, we will soon be storing large files which will not be loaded 
into the database per se,
but would like to retrieve them based on the meta data in BASE2.. thus 
no jobs will be created, unless
i create a bogus plugin importer just to make a file to RawBioAssay 
connection.


it seems less of a hack, but not much, to instead create an annotation 
field named File that describes

the RawBioAssay and place the file path there.

-keith

Nicklas Nordborg wrote:

Charles Girardot wrote:
  

Hi Nicklas,

I might have missed something as I am not using BASE2 now and haven't  
investigated in details. I have a comment on this thread :
Very often people come up with some logics in naming their files and,  
in the context of re-exporting them (eg, submission), they like to  
see file having original names. They might be various other situation  
where having the same name is good. I think you should keep original  
names somewhere like in BASE1, it makes things easier.



When you upload files the original filename is kept in the database. 
There is however no information that links a rawbioassay to the file 
that was used for importing the data. It has nothing to do with the file 
names.


/Nicklas


  

Am I off track?

Cheers

Charles

On Dec 8, 2006, at 9:00 AM, Nicklas Nordborg wrote:



Keith Ching wrote:
  

Hi,

I am trying to write a plugin that will download the original source
files used to
populate the RawBioAssay objects.  When you import data into a  
RawBioAssay,
is the name of the original source file saved somewhere in the  
database?


I am at the point where i can select the RawBioAssays that i want to
download.  It's
just I can't find what file was originally used to create it.


That information is not saved.

/Nicklas


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=
Charles Girardot
European Molecular Biology Laboratory
C. Boulin and E. Furlong Groups
Tel: +49-6221-387-8426
Email: [EMAIL PROTECTED]
Meyerhofstrasse 1 - Postfach 10.2209
69012 Heidelberg, Germany
=



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--
Keith Ching, Ph.D.
Bioinformatics, Laboratory of Gene Regulation
Ludwig Institute for Cancer Research
9500 Gilman Drive, #0653
CMM East, Room 3020
La Jolla, CA 92093-0653

[EMAIL PROTECTED]
858-822-5767

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