[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon 
ah! ok, that's a different matter
j

Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:29
An: Hughes, Jon <jon.hug...@bot3.bio.uni-giessen.de>; CCP4BB@JISCMAIL.AC.UK
Betreff: RE: [ccp4bb] UVEX UV Fluorescence

But I think he was asking about imaging of intrinsic fluorescence of protein 
crystals…

I like your idea about gel imaging, though.

JPK

From: Hughes, Jon [mailto:jon.hug...@bot3.bio.uni-giessen.de]
Sent: Wednesday, April 05, 2017 8:23 AM
To: Keller, Jacob <kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>>; 
CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: AW: [ccp4bb] UVEX UV Fluorescence

for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference 
filter. ccd chips are sensitive to infrared and transilluminators produce a lot 
of it!
best
j

Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:16
An: Hughes, Jon 
<jon.hug...@bot3.bio.uni-giessen.de<mailto:jon.hug...@bot3.bio.uni-giessen.de>>;
 CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: RE: [ccp4bb] UVEX UV Fluorescence

Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a 
typo?

JPK

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, 
Jon
Sent: Wednesday, April 05, 2017 6:43 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

you can save a fortune by just fitting a 590nm interference filter to the front 
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my 
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian 
Cukier
Gesendet: Mittwoch, 5. April 2017 12:26
An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [ccp4bb] UVEX UV Fluorescence

Dear All,

We are about to buy a new imaging system for our laboratories and we consider 
UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about 
this equipment (eg. is it reliable, robust, no technical issues, 
low-maintenance etc.)? All comments will be appreciated.

Thanks,
Cyprian

Re: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Keller, Jacob 
But I think he was asking about imaging of intrinsic fluorescence of protein 
crystals…

I like your idea about gel imaging, though.

JPK

From: Hughes, Jon [mailto:jon.hug...@bot3.bio.uni-giessen.de]
Sent: Wednesday, April 05, 2017 8:23 AM
To: Keller, Jacob <kell...@janelia.hhmi.org>; CCP4BB@JISCMAIL.AC.UK
Subject: AW: [ccp4bb] UVEX UV Fluorescence

for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference 
filter. ccd chips are sensitive to infrared and transilluminators produce a lot 
of it!
best
j

Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:16
An: Hughes, Jon 
<jon.hug...@bot3.bio.uni-giessen.de<mailto:jon.hug...@bot3.bio.uni-giessen.de>>;
 CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: RE: [ccp4bb] UVEX UV Fluorescence

Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a 
typo?

JPK

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, 
Jon
Sent: Wednesday, April 05, 2017 6:43 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

you can save a fortune by just fitting a 590nm interference filter to the front 
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my 
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian 
Cukier
Gesendet: Mittwoch, 5. April 2017 12:26
An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [ccp4bb] UVEX UV Fluorescence

Dear All,

We are about to buy a new imaging system for our laboratories and we consider 
UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about 
this equipment (eg. is it reliable, robust, no technical issues, 
low-maintenance etc.)? All comments will be appreciated.

Thanks,
Cyprian

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon 
for ethidium bromide you need a 590nm (50-100nm FWHH) bandpass interference 
filter. ccd chips are sensitive to infrared and transilluminators produce a lot 
of it!
best
j

Von: Keller, Jacob [mailto:kell...@janelia.hhmi.org]
Gesendet: Mittwoch, 5. April 2017 14:16
An: Hughes, Jon <jon.hug...@bot3.bio.uni-giessen.de>; CCP4BB@JISCMAIL.AC.UK
Betreff: RE: [ccp4bb] UVEX UV Fluorescence

Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a 
typo?

JPK

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, 
Jon
Sent: Wednesday, April 05, 2017 6:43 AM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

you can save a fortune by just fitting a 590nm interference filter to the front 
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my 
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian 
Cukier
Gesendet: Mittwoch, 5. April 2017 12:26
An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [ccp4bb] UVEX UV Fluorescence

Dear All,

We are about to buy a new imaging system for our laboratories and we consider 
UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about 
this equipment (eg. is it reliable, robust, no technical issues, 
low-maintenance etc.)? All comments will be appreciated.

Thanks,
Cyprian

Re: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Keller, Jacob 
Why 590 nm? BP, SP, LP? I would have thought 390 nm LP or similar--was it a 
typo?

JPK

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, 
Jon
Sent: Wednesday, April 05, 2017 6:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

you can save a fortune by just fitting a 590nm interference filter to the front 
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my 
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian 
Cukier
Gesendet: Mittwoch, 5. April 2017 12:26
An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Betreff: [ccp4bb] UVEX UV Fluorescence

Dear All,

We are about to buy a new imaging system for our laboratories and we consider 
UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about 
this equipment (eg. is it reliable, robust, no technical issues, 
low-maintenance etc.)? All comments will be appreciated.

Thanks,
Cyprian

[ccp4bb] AW: [ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Hughes, Jon 
you can save a fortune by just fitting a 590nm interference filter to the front 
of a ccd camera attached to a PC + a UV/B transilluminator. i set this up in my 
lab for about €1000 total 15 years ago and we've used it everyday since.
cheers
jon

Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Cyprian 
Cukier
Gesendet: Mittwoch, 5. April 2017 12:26
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] UVEX UV Fluorescence

Dear All,

We are about to buy a new imaging system for our laboratories and we consider 
UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about 
this equipment (eg. is it reliable, robust, no technical issues, 
low-maintenance etc.)? All comments will be appreciated.

Thanks,
Cyprian

[ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Cyprian Cukier 
Dear All,

 

We are about to buy a new imaging system for our laboratories and we
consider UVEX UV Fluorescence Imaging from MD. Can anyone provide some
comments about this equipment (eg. is it reliable, robust, no technical
issues, low-maintenance etc.)? All comments will be appreciated.

 

Thanks,

Cyprian