[R-sig-phylo] phylogenetic regression and pPCA with intraspecific variability

2013-08-19 Thread Tanja Vukov
Dear all,

I am using pPCA with size corrected traits (obtained by phyl.resid) as a
part of ecomorphology study of 20 salamander’s species (whole process is
done with mean values of traits per species). Few days ago I discovered a
way to test for phylogenetic signal (Blomberg’s K) with intraspecific
variability. I wondering is there a way to do size correction (via
phylogenetic regression) and pPCA taking intraspecific variability into
account (by using multiple observations per species)?


Thank you very in advance!

Kind regards,

Tanja Vukov.


Institute for biological research
Dept. of evolutionary biology
Bulevar despota Stefana 142
11000 Belgrade
Serbia

[[alternative HTML version deleted]]

___
R-sig-phylo mailing list - R-sig-phylo@r-project.org
https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
Searchable archive at http://www.mail-archive.com/r-sig-phylo@r-project.org/

Re: [R-sig-phylo] Rooting and rotating in ape

2013-08-19 Thread Naxerova, Kamila
Sorry, forgot the most important part of R version
[1] R version 2.15.3 (2013-03-01)
On Aug 19, 2013, at 10:12 PM, Liam J. Revell liam.rev...@umb.edu
 wrote:

 Hi Kamila.
 
 1) A tree stored in memory as an object of class phylo can have a root edge 
 - but no root label. One option (if your tree has edge lengths) that 
 approximates what it sounds like you want to do is to re-root the tree 0 
 distance below the tip that is your root. You can do this using the function 
 reroot (which uses root internally) from the phytools package. E.g., to 
 re-root the (random, in this case) tree 'tree' at the tip t10:
 
 library(phytools)
 tree-rtree(n=10) ## just for demonstration
 nn-which(tree$tip.label==t10) ## get node number of tip
 tt-reroot(tree,nn,tree$edge.length[which(tree$edge[,2]==nn)])
 plotTree(tt)
 
 2) To better help you with this, can you give us a reproducible error? (E.g., 
 your tree, the code that produces the error, your version of R and ape.)
 
 All the best, Liam
 
 Liam J. Revell, Assistant Professor of Biology
 University of Massachusetts Boston
 web: http://faculty.umb.edu/liam.revell/
 email: liam.rev...@umb.edu
 blog: http://blog.phytools.org
 
 On 8/19/2013 9:51 PM, Naxerova, Kamila wrote:
 Dear list,
 
 I have just started playing around with ape, and I have some naive newbie 
 questions.  Any help would be very much appreciated.
 
 1) In my data set, the root is extant. Is there a way to directly designate 
 one of the taxa as the root?
 2) When I use the rotate function on what I think is a fairly small and 
 simple tree, R always freezes and I have to kill it and start over. Any 
 ideas what I might be doing wrong?
 
 Many thanks in advance.
 Kamila
 ___
 R-sig-phylo mailing list - R-sig-phylo@r-project.org
 https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
 Searchable archive at http://www.mail-archive.com/r-sig-phylo@r-project.org/
 

___
R-sig-phylo mailing list - R-sig-phylo@r-project.org
https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
Searchable archive at http://www.mail-archive.com/r-sig-phylo@r-project.org/


Re: [R-sig-phylo] Rooting and rotating in ape

2013-08-19 Thread Liam J. Revell

Hi Kamila.

The only two tricks I can suggest are:

tree-reorder(reorder(tree,pruningwise))

## or

tree-read.tree(text=write.tree(tree))

Sometimes functions in ape  other packages might produce phylo 
objects as output that have a nonstandard edge order  these steps can 
often be used to resolve that issue.


Let us know if this helps. If not, you could send the tree in Newick 
format as a text file. You can easily strip the tip numbers if you are 
concerned about distributing data in progress, for example:


temp-tree
temp$tip.label-as.character(1:length(tree$tip.label))
## tree 'temp' now has numbers as labels
write.tree(temp,file=filename)

All the best, Liam

Liam J. Revell, Assistant Professor of Biology
University of Massachusetts Boston
web: http://faculty.umb.edu/liam.revell/
email: liam.rev...@umb.edu
blog: http://blog.phytools.org

On 8/19/2013 10:43 PM, Naxerova, Kamila wrote:

Hi Liam,

thank you so much!

Your answer to 1) is exactly what I wanted to do. My root is not hypothetical, but one of 
the samples in my data set. I suppose it would also be possible to root by using this 
real root as an outgroup, but it seems a bit counterintuitive, no? Apparently 
having an extant root is a bit of an unusual situation? I like the solution you suggested 
a lot.

2)

R.Version()

$platform
[1] i386-apple-darwin9.8.0
$arch
[1] i386
$os
[1] darwin9.8.0

ape version 3.0-8

I am not entirely sure what you mean by providing the tree -- it's a tree I 
made in ape with nj() on a large distance matrix -- not sure what the best way of sharing 
this structure on the list would be? Plotting it with default values is no problem. I 
then use nodelabels() to identify which nodes I want to rotate around and say something 
like plot(rotate(tree,node)) -- that's when R freezes. I have tried to do the same thing 
with random trees generated by rtree, and it worked fine.

Thanks again.
Kamila

On Aug 19, 2013, at 10:12 PM, Liam J. Revell liam.rev...@umb.edu
  wrote:


Hi Kamila.

1) A tree stored in memory as an object of class phylo can have a root edge - but no 
root label. One option (if your tree has edge lengths) that approximates what it sounds like you 
want to do is to re-root the tree 0 distance below the tip that is your root. You can do this using 
the function reroot (which uses root internally) from the phytools package. E.g., to re-root the 
(random, in this case) tree 'tree' at the tip t10:

library(phytools)
tree-rtree(n=10) ## just for demonstration
nn-which(tree$tip.label==t10) ## get node number of tip
tt-reroot(tree,nn,tree$edge.length[which(tree$edge[,2]==nn)])
plotTree(tt)

2) To better help you with this, can you give us a reproducible error? (E.g., 
your tree, the code that produces the error, your version of R and ape.)

All the best, Liam

Liam J. Revell, Assistant Professor of Biology
University of Massachusetts Boston
web: http://faculty.umb.edu/liam.revell/
email: liam.rev...@umb.edu
blog: http://blog.phytools.org

On 8/19/2013 9:51 PM, Naxerova, Kamila wrote:

Dear list,

I have just started playing around with ape, and I have some naive newbie 
questions.  Any help would be very much appreciated.

1) In my data set, the root is extant. Is there a way to directly designate one 
of the taxa as the root?
2) When I use the rotate function on what I think is a fairly small and simple 
tree, R always freezes and I have to kill it and start over. Any ideas what I 
might be doing wrong?

Many thanks in advance.
Kamila
___
R-sig-phylo mailing list - R-sig-phylo@r-project.org
https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
Searchable archive at http://www.mail-archive.com/r-sig-phylo@r-project.org/





___
R-sig-phylo mailing list - R-sig-phylo@r-project.org
https://stat.ethz.ch/mailman/listinfo/r-sig-phylo
Searchable archive at http://www.mail-archive.com/r-sig-phylo@r-project.org/