Hi Amlan,
given that it should not depend on which school of thought you follow:
2*(Fo-Fc) + Fc vs Fo + (Fo-Fc), you should be able to see the same thing at
appropriately chosen contouring levels in both maps, I think... (I could be
wrong at this time of the night, of course!)
Pavel
On Mon,
Dear Amlan,
The sigma of an Fo-Fc map map depends on the residual noise in your map. In a
well-refined structure, the sigma will be low, so at 3 sigma it will show very
weak features.
My guess is that your ligand is present in partial occupancy and that you will
find it in your 2Fo-Fc map when
Dear everybody,
to my recent experience not everything is good in the stereo world. Since I see
that others don't have these problems (and I am happy/sad to see that the same
exact combinations work without problems) I would just like to add my
experience.
For the past 4 months I have been
*Postdoctoral position in structure-function studies of G-protein
networks in bacterial virulence and persistence*
*Structural Biology Brussels (Vrije Universiteit Brussel / VIB) --
Brussels, Belgium*
**
*At the Structural Biology Brussels laboratory (Vrije Universiteit
Brussel / VIB),
Hi Matic,
I'm using a Quadro 600 on an Asus VG278HR screen on RHEL 6.3. All is
golden. I created xorg.conf (below) by running nvidia-xconfig after
installation of the Nvidia driver. (Recent Nvidia drivers are buggy and
you'll have to wear your stereo glasses upside down from time to time
Dear CCP4 Users,
An update for the CCP4-6.4.0 series has just been released, consisting of the
following changes
◆ ipmosflm(windows)
- update to 7.1.0
◆ imosflm(all platforms)
- fix for MOSFLM_EXEC not being set under csh
- fix for false missing ipmosflm error
Note that auto-updates work only
Colleagues,
Some years ago, Ray Stevens published a nice paper on a cysteine-reactive dye
(CPM) that could be used in a Thermofluor-type thermal shift assay for membrane
proteins. (The fluorescence of the ANS dyes, used for soluble proteins,
increases with low dielectric, which is fine for
I'm pleased to announce the release of DSSR v1.0.3, a software program for
Defining the Secondary Structures of RNA from three-dimensional coordinates
(http://goo.gl/ap12xJ). Since its initial beta release in early March 2013,
I've continuously refined the program by taking user feedback into
