Re: [ccp4bb] preparing DL-Malic acid stock solution

The way I have done it (and it was sort of fun in a mad scientist way) was to mix up solid DL-malic acid and sodium hydroxide in the right amounts and add water as needed. This generates of heat, but gets around the solubility minimum which is impossible to get out of: I mixed 40.23 g of

[ccp4bb] Off topic but of interest to SSRL users

Dear All, For those that have Facebook accounts the SSRL Users Organization has established a Facebook page at https://www.facebook.com/ssrluec/. This has details on the SSRL/LCLS users meeting and news stories coming from SSRL. Recently you may or may not have heard that the US Department of

Re: [ccp4bb] preparing DL-Malic acid stock solution

(di)Sodium D,L-malate is available from Sigma-Aldrich (# CDS023113) and probably dissolves to give a 3M solution which is slightly alkaline. If pK2 is 5.1, then an insignificant amt of malic acid should bring the pH down to 7 (If you have to add a significant amount, just add more water to dilute

Re: [ccp4bb] preparing DL-Malic acid stock solution

As the pKa1 of malic acid is 3.4, it may be that the partially neutralized malic acid salt is less soluble than the acid or the fully neutralized salt. The pKa2 of malic acid is 5.1. Contact the people at Hampton Research https://www.hamptonresearch.com/contact_us.aspx and ask them. They sell

[ccp4bb] preparing DL-Malic acid stock solution

Dear all, we’ve recently having trouble preparing a 3 M stock solution of DL-Malic Acid, pH 7 (which we had, so it’s doable!). When we reach pH 3 - 4 the solution turns milky white and does not goes back to a clear solution even when the pH is raised. Does anybody have any advice on how to get

[ccp4bb] Postdoctoral position in time-resolved EM at the University of Leeds

Dear All, A BBSRC funded postdoctoral position is available to become part of an international team working on new methodologies for time resolved single particle cryo-EM allowing us to trap different conformational states of proteins and protein complexes in the µs-ms

Re: [ccp4bb] AutoSol invalid MTZ column_types error

?Hi Mintu, I will answer you on the Phenix mailing list! All the best, Tom T From: CCP4 bulletin board on behalf of Mintu Chandra Sent: Wednesday, June 7, 2017 9:33 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb]

Re: [ccp4bb] Problems with an exonuclease

Dear all, I apologize that till now I was making the mistake of not sending the replies in the common thread, but somehow only to each person individually. I am summarizing my answers since yesterday here: I have tried refolding it at pH 7, 7.5 and 8. I add 4mM TCEP to my urea buffers and reduce

[ccp4bb] Call for group leader et IPBS, Toulouse, France

Dear CCP4ers, The Institute of Pharmacology and Structural Biology (IPBS) in Toulouse, France, is seeking new group leaders. Details of the call are available at http://www.ipbs.fr/call2017. Please note that the application deadline is July 15, 2017. Address inquiries related to the call at: