On 19/03/2018 21:18, Nikolas wrote:
I'm solving my first structure ever (yay!)
Yay!
on a MAC OS
Boo!
and they keybindings doesn't work.
Boo.
I thought that
they would come with the CCP4 suite but I've found also Paul's and Bernhard's files on COOT wiki and tried
to create the
Dear Professor Ethan,
I apologize, for the partial
information provided by me in the second email. For the *in vivo* assembled
(as-purified) protein, we have confirmed the [4Fe-4S] cluster by CD and
EPR. From the 415/280 ratio and Ferrozine assay, we have
On Monday, March 19, 2018 2:20:50 PM PDT PULSARSTRIAN wrote:
> Dear Professor Diana,
>
> Thank you very much for your
> comment, and we will certainly look at, if ‘domain swapping’ is any reason
> for the trimeric nature of the protein.
>
>
Hello everyone,
I'm solving my first structure ever (yay!) on a MAC OS and they keybindings
doesn't work. I thought that they would come with the CCP4 suite but I've
found also Paul's and Bernhard's files on COOT wiki and tried to create the
.coot file but it didn't work. Unfortunately I'm a
Dear Professor Diana,
Thank you very much for your
comment, and we will certainly look at, if ‘domain swapping’ is any reason
for the trimeric nature of the protein.
There are two cysteines in helix-1 and two cysteines
in helix 3.
Is it possible that you have a case of domain swapping that causes the trimeric
assembly?
Diana
**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Rm.
Dear all,
Sorry for the slightly off-topic question.
I am working on a non-native, *de novo* [4Fe-4S]
protein, designed as a four-helix bundle. The *in vitro* reconstituted
protein assembles with [4Fe-4S] (confirmed by EPR) and exists in
monomer-dimer
ReNaFoBiS. Oleron 2018. There are only few places left.
The 5th RéNaFoBiS Oléron workshop, dedicated to Integrative Structural Biology,
will take place on the Oléron island (France), from June the 1st until June the
8th, 2018. The main objective of this workshop is to offer a theoretical and
Dear all,
Further to lasts week message...:
1) Mea culpa... to my shame I forget to include 'Instruct' in the title of
the workshop. They are kindly supporting this workshop.
2) Places to register interest are filling up rapidly so if you would like
to come please register soon:
Dear All,
Thanks everyone for the suggestions and clarifications. As Eleanor and Garib
wrote, I cannot find real differences in map quality or statistics after
refinement depending if I set or not the resolution edges.
This is more a matter of how the remarks in the pdb are recorded for
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