As for your second question, whether refining in a lower symmetry affects the
R-factors,
On 7/20/22 10:10 AM, Jorge Iulek wrote:
Related, and a question I mentioned before in this forum: what if a
genuine 2 axis (say , P222 to P2, or even to P1) (I do not mean this is the
case here) is
Thanks Kay and Ralf for your input.
I will check out the reference pointed and search for more.
Best wishes,
Jorge
Forwarded Message
Subject:symmetry possibilities
Date: Wed, 20 Jul 2022 11:10:04 -0300
From: Jorge Iulek
To: CCP4BB@JISCMAIL.AC.UK
Dear
The Choi laboratory is moving to Indiana University, Bloomington, IN,
and multiple post-doctoral positions are available. We study the structure and
mechanism of viral replication and infection machinery focusing on RNA viruses.
In particular, we are interested in RNA-protein
This presentation might help.
The live webinar is over but you might find the recorded version sometime time
later. Good luck
John
Begin forwarded message:
> From: Zoom
> Date: July 20, 2022 at 6:13:22 AM HST
> To: "j.hasty"
> Subject: Reminder: CryoEM Centers Webinar Series starts in 1 day
Yes - I wondered how much data can be extracted from each crystal..
Eleanor
On Fri, 22 Jul 2022 at 19:23, Kay Diederichs
wrote:
> Hi Eleanor,
>
> yes, for sufficiently complete datasets a reference dataset is enough.
> But in serial crystallography, there is typically little overlap between
>
I don't have a particular solution in mind, but wish to note that,
in my experience, something like this near a ASN or ASP residue is not
"unusual electron density". I have seen ugly density that looks like
this many times. I usually end up with partially occupied water
molecules, but am
Hi Eleanor,
yes, for sufficiently complete datasets a reference dataset is enough.
But in serial crystallography, there is typically little overlap between
individual data sets. And the data quality is often low.
In my posting I forgot to say that CrystFEL also has a facility to overcome
Surely once you have indexed one crystal, you can use the facility to check
the next ones indexing against the reference - aka pointless?
On Fri, 22 Jul 2022 at 16:20, Kay Diederichs
wrote:
> Hi Monika,
>
> in June we had a summer school at MaxIV, and one of the topics was serial
>
Fully funded postdoctoral positions are available in the laboratory of Prof.
Ian Wilson at Scripps Research (http://wilson.scripps.edu, email:
wil...@scripps.edu) for highly motivated candidates who are interested in
immune recognition and inhibition of microbial pathogens. Specifically, we
Hi Monika,
in June we had a summer school at MaxIV, and one of the topics was serial
crystallography - with lectures and tutorials. Maybe you can talk to the people
who attended the course, and the organizers, Ana Gonzalez and Thomas Ursby, and
ask them for help.
It is more difficult to
Jens has passed away. Please cancel whatever account he has and/or remove
his email from your list. Thank you
*++ *
*Jens J. Birktoft *
*e-mail: jens.kn...@gmail.com ; Phone: 212-749-5057 *
*slow-mail: 350 Central Park West, Suite 9F, New York, NY
Our group has an opening for a postdoctoral fellow to be funded on a Cancer
Research UK programme aimed at understanding lysosomal control of cell
proliferation and stress responses in cancer cells. The programme involves
cryo-electron microscopy, X-ray crystallography, H/D exchange mass
Dear all,
we have an attractive opportunity for a membrane protein biochemist and
structural biologist in the group of Herbert Nar at Boehringer Ingelheim. The
work is focused on pharmaceutically relevant integral membrane proteins with
the goal to elucidate the 3D structure, study ligand
Most molecular replacement programs allow you to search in all possible
space groups consistent with a given point group.
Eleanor
On Fri, 22 Jul 2022 at 11:18, Schreuder, Herman /DE <
herman.schreu...@sanofi.com> wrote:
> Hi Monika,
>
>
>
> I would process the data using the point group
Hmmm - is there a smaller lattice which might fit the density? As Andrew
says there are examples of pseudo emptiness in crystals but there are more
examples of wrong lattices!
Check for non-crystallographic translation?
You could attach the pointless/aimless/etc log files..
Eleanor
On Fri, 22 Jul
Hi Monika,
I would process the data using the point group information ignoring any
possible screw axes, e.g. in space group P222, where the true space group might
be P212121.
The next step depends on how you plan to solve the structure. You mention that
there is no cryo structure, do you mean
Hi,
I’m hoping someone can help me how to determine a space group from my
collection.
I did serial crystallography on a crystal that doesn’t have a cryo structure. I
was able to determine the Point group but for the next step I’m stuck.
Kind regards,
Monika Bjelcic
PhD student at BioMAX
Dear Florian,
There have been previous examples where there are distinct
“layers” of protein packing without any obvious density linking the layers, as
it appears in the image you show. One is tyrosyl tRNA synthetase, where the
layers were in fact linked by a disordered
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