Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

Gosh Emmanual, your email is one amazing example of what the problem is, every single paragraph - thanks for putting that one out! My last email for this subject, I promise. Thanks a lot for the off-list feedbacks! Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

guys to get things right in ways we did not we have to create alternative perspectives and come up with new forms of community work. Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail: baerbel.bl...@intherabio.com Phone: +41 43

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

Hey Susan, I totally agree with you and Pietro. Here's an idea - why don't you organise a structural biology workshop with a females-only speaker list? Don't give it some stupid feminist name and don't exclude male participants. Also don't exclude male speakers deliberately - that would be

[ccp4bb] Off-topic: (micro)array image analysis software

, could point me to a program that runs on a Mac and is suitable for array analysis (it does not actually have to be free as long as it works for users who do not write code). I do have a GAL file for the images. Many thanks for your help and sorry for the spam! Bärbel -- Bärbel

Re: [ccp4bb] Questionable Ligand Density: 6MO0, 6MO1, 6MO2

with editors from glossy journals we should not assume bad intentions by all the authors as a group. So let’s see what he says. Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail: baerbel.bl...@intherabio.com Phone: +41 43

Re: [ccp4bb] Questionable Ligand Density: 6MO0, 6MO1, 6MO2

Krishna Murthy structures, right? The associated (now retracted) article I mean is here https://www.sciencedirect.com/science/article/pii/S002228360093924X?via%3Dihub Kind regards, Bärbel --- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail

Re: [ccp4bb] challenges in structural biology

ther than structural biology I am under the stark impression that some students have indeed already started to regard crystallography as a dinosaur technique, and not just because cryoEM improved - that is a challenge to structural biology, too. Kind regards, Bärbel -- Bärbel Blaum, PhD I

Re: [ccp4bb] analytical gel filtration columns

luck, Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail: baerbel.bl...@intherabio.com Phone: +41 43 477 94 72-- Von: CCP4 bulletin board im Auftrag von David Blum Antworten an: David Blum Datum: Mittwoch, 17

[ccp4bb] Nanodrop-1000 lamp

Dear all, does anyone happen to know exactly which Xe flash lamp is (or better: was) being used in the discontinued Nanodrop-1000? Thanks for your help! Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail

Re: [ccp4bb] AW: COOT: adding xylose to a plant glycosylation chain

o23Y90cq7kVyr8vdELWgK8qH3eZCA75PrDFpQ=> To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse

Re: [ccp4bb] "Atomic resolution"

les with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 733 48 Raum 311

Re: [ccp4bb] Unbranched polysaccharide chain

rtment of Chemistry City College of New York 85 Saint Nicholas Terrace, CDI 2.318 New York, NY 10031 http://www.khayatlab.org/ 212-650-6070 -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 733 48 Raum 311

Re: [ccp4bb] Sugar-Pi-stacking

lamt.org/ b...@hofkristallamt.org <mailto:b...@hofkristallamt.org> +1 925 209 7429 +43 767 571 0536 -- All models are wrong but some are useful. ------ -- Bärbel Blaum, Ph.D. Interfakultäres Inst

Re: [ccp4bb] Best method for carbohydrate refinement

glycolisation, identify the most probable ones, determine the restrictions for each and refine them. I could do it by hand on every refinement cycle (or a couple of cycles), but for many datasets it would take ages. What protocol would you suggest? I really appreciate any help in this subjec

Re: [ccp4bb] Cleaved peptide density!

Oslo, Norway -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 75 359 http://www.ifib.uni-tuebingen.de/research/blaum.html

Re: [ccp4bb] Crystallization of a minority fraction monomers

us immediately by reply e-mail and then delete it from your system. -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 75 359 http://www.ifib.uni-tuebingen.de/research/blaum.html

Re: [ccp4bb] Trimming of carbohydrate chains

expression yields and poorly soluble protein, so I would like to try to remove some, but not all carbohydrate. Does anyone has a good protocol to trim, but not completely remove the carbohydrate? Thank you, Herman Schreuder -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie

Re: [ccp4bb] How to make covalent bond between sugar monomers?

. -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 73 375

Re: [ccp4bb] PDB passes 100,000 structure milestone

for a while has a list of dodgy structures that they think should be retracted - not always with justification. -Nat -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 73 375

Re: [ccp4bb] docking program

at protein-DNA interactions? -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 73 375

Re: [ccp4bb] Examples of multiple ASU copies with different conformations

in the ASU, with dramatically different domain organization. I'd like to find some additional examples - can anyone suggest some other structures that have multiple copies with large structural variations? Thanks in advance! Shane Caldwell McGill University -- Bärbel Blaum, Ph.D

Re: [ccp4bb] distinguish ligand binding sites within a protein

relationships dissolve then respect and devotion arise; When a nation falls to chaos then loyalty and patriotism are born. -- / Lao Tse / -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71

Re: [ccp4bb] distinguish ligand binding sites within a protein

, it is not very likely given that affinity gap is sufficient to be observable by ITC. Mutagenesis is a good option too. On Tue, 2013-11-19 at 17:12 +0100, Bärbel Blaum wrote: Hello, we work with proteins that have typically several chemically identical binding sites (viral capsid proteins fully assembled

Re: [ccp4bb] Off-topic: Correlation of crystallographic B factors with NMR relaxation parameters

measurements of NMR relaxation parameters? Regards, Florian -- Bärbel Blaum, Ph.D. Interfakultäres Institut für Biochemie (IFIB) Hoppe-Seyler-Strasse 4 D-72076 Tübingen Germany +49 70 71 29 73 375