Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

protein goes brown after a nickel column and you thought it was something special to your protein, try again..! If your protein can’t handle EDTA, try the pH trick suggested in another post. If none of that works, then move to GST or some such… Ed https://www.ularkin.org/team-member/thomas-edwards

[ccp4bb] FW: Postdoc position in Structure-Based Cancer Drug Discovery - Astbury Centre, Leeds University

PLEASE DO NOT REPLY TO ME... Please direct enquiries to Prof Alex Breeze: E: a.l.bre...@leeds.ac.uk T: +44 113 343 0087 Dear Colleagues, ***Postdoctoral Research Fellow in Structure-Based Cancer Drug Discovery*** There is an immediate opening in my lab at

Re: [ccp4bb] [OT] Structure-related pun needed urgently

“Can I speed up structure solution using different phases”…?? I’ll get my coat. [Gerard - what is the audience? Crystallographers vs general public might make a difference..!] Ed T.A.Edwards Ph.D. Associate Professor of Biochemistry Deputy Head of School _

[ccp4bb] Rigaku system up for grabs

Dear CCP4bb We are going through a large lab refurbishment programme and that, along with space costings for facilities, mean that sadly we will not be able to keep our local generator & detector. If anybody can come to Leeds and take it all away (free!) then please do so! The system: Rigaku

Re: [ccp4bb] OFF TOPIC

You don’t say so, but one assumes that you have a BAP tag on the protein, and co-express a biotin ligase such as BirA? Ed T.A.Edwards Ph.D. Associate Professor of Biochemistry Deputy Head of School _ Astbury Centre for Structural Molecular Biology School of

[ccp4bb] Asbury Conversation April 16-17th. Abstract deadline approaching

Dear Structural Molecular Biologists, There are still some places available to register for the Astbury Conversation, so if you or any colleagues who would benefit from coming – full details are available on the web site http://www.astburyconversation.leeds.ac.uk/ With just over eight weeks

Re: [ccp4bb] "Atomic resolution"

Dear Jacob, Ah... this old chestnut! Current EM people say that they are at atomic resolution because they are building atomic models (naive??). I have been criticised in the past for using the term with say 2.2A diffraction data. By co-authors and reviewers alike. When I was young and naive.

[ccp4bb] structural biologist to work on cancer drug discovery

Please respond direct to Alex. Dear Colleagues, I have a 16 month postdoctoral position available immediately in my lab at the Astbury Centre, University of Leeds, for a versatile structural biologist to work on cancer drug discovery in a collaboration with the Northern Institute for Cancer

[ccp4bb] registration for the Astbury Conversation 2018 is now open

Dear CCP4 members, Following the huge success of the inaugural Astbury Conversation 2016, we are delighted to announce that registration for the Astbury Conversation 2018 is now open!! The event will bring together some of the world’s most prominent molecular/chemical/structural biologists

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

A few tips, some/all of which may be relevant. Or not… If you are in 96 or 384 well plates, they vary. We tried quite a few batches and brands before settling on one. Keep your salt concentration as low as possible. You should be able to measure Kd in a range of about 1nM to low uM. Outside

[ccp4bb] 15 positions for Early Stage Researchers (ESR) in virology

As part of the “HONOURS” ITN EU network, there are 15 early stage researcher (studying for a PhD) positions available in Belgium, Germany, the Netherlands, Spain, Switzerland and the United Kingdom. See details below, and specific details of those hosted at the University of Leeds. Ed

[ccp4bb] Research Fellow in Chemical Biology, Leeds

Dear CCP4 members, A reminder of the 30th March closing date for a research fellow position in my lab to collaborate with chemists here at Leeds to design inhibitors of protein-protein interactions: http://jobs.leeds.ac.uk/FBSAS1006 Closing Date: Thursday 30 March 2017 We are looking for

[ccp4bb] Research Fellow in Structural/Chemical Biology

Details below of a position now open in my lab as part of a project to design and test inhibitors of protein-protein interactions. Please Email me with any informal enquiries. Research Fellow in Structural Molecular Biology Are you looking to apply your skills in Structural Biology to the

Re: [ccp4bb] metal ion coordination

environments present in protein structures. http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18614239 Tom Thomas Edwards, Ph.D. Director, Technology Client Solutions Co-PI, Seattle Structural Genomics Center for Infectious Disease (SSGCID) Head, Infectious Disease Practice Area Emerald Bio Office: (206

[ccp4bb] Lecture in X-ray crystallography, University of Leeds

Please see the link below for an advert for a new Lectureship in Macromolecular X-Ray Crystallography at the University of Leeds. http://jobs.leeds.ac.uk/fe/tpl_universityofleeds01.asp?s=4A515F4E5A565B1Ajobid=110947,6951214874key=11037106c=612123217223pagestamp=sepwjldjailxhilecl Job Summary

[ccp4bb] Bper lysis reagent recipe

Lovely CCP4BB Members, Hopefully not too far off topic: It appears that our sonicator is, ironically, bust. Whilst I wait for a new one, and/or some B-PER reagent, does anybody have a home made detergent lysis reagent (something like B-PER) recipe? Only E. coli to bust open (for now). Many

[ccp4bb] post-doctoral opportunity at the University of Leeds

...@leeds.ac.ukmailto:j.n.b...@leeds.ac.uk or Dr Thomas Edwards, email t.a.edwa...@leeds.ac.ukmailto:t.a.edwa...@leeds.ac.uk. Closing Date: 13 February 2014 http://jobs.leeds.ac.uk/fe/tpl_universityofleeds01.asp?s=4A515F4E5A565B1Ajobid=108922,2351796198key=9056137c=344787029814pagestamp=sedylihlceulaesvjp Ed

Re: [ccp4bb] Examples of multiple ASU copies with different conformations

in the opposite direction between the two molecules in the asymmetric unit. See Figure 2C of the paper: Robertson, M.P. Scott, W.G. The structural basis of ribozyme-catalyzed RNA assembly. Science 2007, 315, 1549-1553; PubMed ID 17363667. Only one conformation appears to be active. Thomas Edwards From

[ccp4bb] PhD studentship opportunities in Leeds

Dear BB, If you are, or know, a keen, talented, motivated student looking for a PhD, then there are several opportunities within the Faculty of Biological Sciences at the University of Leeds. One project involves the use of stapled peptides to investigate inhibition of protein-protein

[ccp4bb] PPI-Net Young Researchers Meeting

Dear CCP4bb, A symposium on protein-protein interactions organised by young researchers from around the UK. Will probably appeal to some from the UK, but obviously all welcome. Please respond to the organising committee (yr2...@ppi-net.org) rather than to me. Thanks Ed This symposium aims

[ccp4bb] protein stain, B-PER

Dear BB, Apologies for being mildly off topic. Maybe. 1. We are trying to express (in E. coli) a protein which appears to be quite sensitive to mechanical disruption. We have ordered some B-PER (Pierce - B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein

[ccp4bb] PhD studentship in Structural Molecular Biology at Leeds Oct 2011

://www.bmb.leeds.ac.uk/staff/tae/AZ_Studentship_inStrucBiol_Ad.pdf For details. Supervisory team: Dr Thomas Edwards, Dr Andy Wilson, Professor Adam Nelson and Dr Stuart Warriner. Protein−protein interactions (PPIs) mediate many biological mechanisms, and, potentially, represent exciting targets

[ccp4bb] Free R with doubled cell edge

Dear BB, Thanks for all of your helpful and useful comments Whilst there was some agreement that simulated annealing should be enough to solve any problems, we have taken perhaps the cautious approach. We have produced a freeR set in the bigger unit cell using reindex as suggested by Eleanor:

[ccp4bb] Free R with doubled cell edge

Dear BB Sages, I have a problem where I think I could very easily do the wrong thing. And I don't really want to do that... We have solved a new structure using zinc SAD phases (1 zinc in 27kD, 2 Zn/AU - Shelx, RESOLVE, ARPwARP. Cool.). In p21 30 109 65 90 105 90 at 2.5A However, we have now

[ccp4bb] Postdoctoral fellowship available at the University of Leeds

A Wellcome Trust funded post-doctoral position is available from September in the Astbury Centre for Structural Molecular Biology at the University of Leeds in the laboratories of Dr John Barr and Dr T. Edwards. The successful applicant will study structural and functional aspects of bunyavirus

Re: [ccp4bb] control of nucleation

Dear Zq, A few ideas: 1) Vary protein concentration, temperature, or protein : mother liquor ratio. 2) Try dioxane - it is supposed to reduce nucleation. 3) give your protein a good hard spin before you set up drops to remove aggregates. 4) seeded factorial screen. 5) re-purify on gel

[ccp4bb] ERC funded Project Studentship in Structural Molecular Biology

Dear Crystallographers... We have an EU funded studentship to study α-Helix Mimetics as Inhibitors of Protein-Protein Interactions Involved in Cancer Development and Progression. Please forward to anybody who may be interested. The purpose of this project is to develop a RULE-BASED APPROACH for

[ccp4bb] Yorkshire Cancer Research funded studentship, University of Leeds

Dear BB, We have a funded PhD studentship position that we must fill with some urgency (the grant must start by 1st Feb 2010). Please see details below, and this link: http://www.bmb.leeds.ac.uk/staff/tae/YCR_advert.pdf This is a collaboration between the Department of Chemistry and the Faculty

Re: [ccp4bb] nad woes

Rob, Thanks for the tips of wisdom. Is the 3 letter code for NADH something different, or is it NAD as well? Ditto for FMN and its reduced form.Thomas Edwards-CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK wrote: -To: CCP4BB@JISCMAIL.AC.UKFrom: Rob Meijers ccp4spama...@yahoo.comSent by: CCP4

[ccp4bb] phased MR

Dear BB, I would like to ask for some advice on phased molecular replacement if possible. I have a MR model that has so far not proved successful with Phaser, Molrep, Amore, Beast etc. I have SeMet MAD data to 3.6A that gives decent looking anomalous difference peaks, looks stable in

[ccp4bb] DLS options?

Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option).

[ccp4bb] imosflm gets stuck - Mac OS 10.5.2

Dear BB, Trying to install imosflm on my new Mac Pro running OS 10.5.2 imosflm will load the images, identify spots on the first frame, says it is trying to find spots on the last frame but then goes into spinning beachball mode and nothing happens. There is some data written out to mosflm.lp

Re: [ccp4bb] imosflm gets stuck - Mac OS 10.5.2 - solved.

Dear Harry, problem solved. You are a gentleman, sir! Thanks Ed -Original Message- From: Harry Powell [mailto:[EMAIL PROTECTED] Sent: Mon 4/14/2008 5:57 PM To: Thomas Edwards Cc: [EMAIL PROTECTED] Subject: Re: [ccp4bb] imosflm gets stuck - Mac OS 10.5.2 Hi Ed just a thought

Re: [ccp4bb] MolRep of coiled coils

Sorry - I should have added that, yes, there are 2 identical peptide chains that should be parallel coiled-coil. Any advice gratefully received. Ed -Original Message- From: cockburn [mailto:[EMAIL PROTECTED] Sent: Fri 3/28/2008 1:35 PM To: Thomas Edwards Subject: Re: [ccp4bb] MolRep

[ccp4bb] FW: polarrfn, pltdev - problem solved

was - other CCP4 things are running fine on the Suse box. For the moment, I have peaks and I guess I will ignore the problem and happily, blindly, carry on... Thanks Ed -Original Message- From: Ian Tickle [mailto:[EMAIL PROTECTED] Sent: Thu 3/6/2008 1:34 PM To: Thomas Edwards Cc: ccp4bb Subject

[ccp4bb] Post-doctoral position Edwards lab University of Leeds

Closing date 15/6/07 We are seeking outstanding and motivated candidates for a postdoctoral position in the laboratory of Dr Thomas Edwards within the Astbury Centre for Structural Molecular Biology at the University of Leeds. The focus of the project is the structure and function of proteins

Re: [ccp4bb] Unusual Difference Fourier near Methionines

Dear Ethayathulla , did you solve the structure by SeMet MAD or some other way? Sometimes you get strange densities around the ends of methionine side chains (around the S/Se) when switching from SeMet data to native. Cheers Ed __ T.Edwards Ph.D. Astbury Centre for