Hi Shane, One of my favorite examples of large structural differences between molecules in the asymmetric unit have is the crystal structure of the L1 ribozyme (PDB ID 2oiu). The Q and P chains have two stems which crudely co-axially stack and a third stem which appears 180 degrees in the opposite direction between the two molecules in the asymmetric unit. See Figure 2C of the paper: Robertson, M.P. & Scott, W.G. The structural basis of ribozyme-catalyzed RNA assembly. Science 2007, 315, 1549-1553; PubMed ID 17363667. Only one conformation appears to be active.
Thomas Edwards From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Aaron Thompson Sent: Monday, January 27, 2014 5:11 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Examples of multiple ASU copies with different conformations The structure of kappa opioid receptor fused with T4 lysozyme (4DJH) contains two copies in the ASU - each copy displays a different orientation between the receptor and lysozyme. On Mon, Jan 27, 2014 at 10:08 AM, Shane Caldwell <shane.caldwel...@gmail.com<mailto:shane.caldwel...@gmail.com>> wrote: Hi ccp4bb, I'm putting together a talk for some peers that highlights strengths and weaknesses of structural models for the outsider. For one point, I'd like to find some examples of proteins that show very different conformations between different copies in the ASU. One example I know of is c-Abl (1OPL), which crystallizes with both autoinhibited and active forms in the ASU, with dramatically different domain organization. I'd like to find some additional examples - can anyone suggest some other structures that have multiple copies with large structural variations? Thanks in advance! Shane Caldwell McGill University
