Hello,
I am working on DSF to verify if some compounds bind to my protein. I see a
negative shift of about 3-4 degrees upon ligand addition (dose-response) in
comparison to the protein alone. I assume that this might be due to the
binding of compound to the unfolded stated rather than folded
Hello All,
This is an off-topic question. I have some issues regarding Fluorescence
Polarization competitive displacement assay and would need some advice.
I have developed an in vitro fluorescence polarization based assay using a
N-terminus labelled FITC peptide. The peptide is 21 amino acids
