We have limited experience in protein:DNA crystallization (N=1), but this is
what worked for us a few years ago.
Like others have advised, I also suggest using oligos with different ends. We
tested two protein constructs, one with a a TEVP cleavable N-terminal His tag
and the other with a
. Trial and error, as usual.
Mark
-Original Message-
From: Acoot Brett acootbr...@yahoo.com
To: CCP4BB CCP4BB@JISCMAIL.AC.UK
Sent: Fri, Jan 3, 2014 9:23 pm
Subject: Re: [ccp4bb] DNA Protein co- Crystallization
Dear All,
For the question, I think for a protein-DNA interaction, the protein
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very
precious on the following queries.
1. My protein is 646 amino acid long and it exists as homodimer. It is also
having around 20 amino acid
Of
venkatareddy dadireddy
Sent: 03 January 2014 11:16
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] DNA Protein co- Crystallization
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very precious
on the following
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact with any sequences of DNA, which will give a lot of combination of
protein-DNA sequence for crystallization screening. Or do anyone regard to
just try the crystallization of the protein-one specific
Dear All,
Thank you very much for your valuable suggestions.
Venkat
On Sat, Jan 4, 2014 at 9:53 AM, Acoot Brett acootbr...@yahoo.com wrote:
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact with any sequences of DNA, which will give a lot of
