On Fri, 17 May 2024 08:27:02 -0700, James Holton wrote:
...
>
>With a bit of a caveat on how many photons/pixel you need for stable
>background subtraction. XDS starts having issues around 1 photon/pixel
>or less, and DIALS claims to be able to get to 0.01 photons/pixel, but I
>have not
Briony
From: CCP4 bulletin board on behalf of Guillaume
Gaullier
Date: Friday, 17 May 2024 at 17:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection
etc. tips
CAUTION: External Message. Use caution opening links and attachments.
Hello,
do now?
Cheers,
Guillaume
From: CCP4 bulletin board on behalf of James Holton
Sent: Friday, May 17, 2024 5:27:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection
etc. tips
A few follow-up questions I g
A few follow-up questions I got out-of-band:
how did you get to the 1:1 relationship between Bijvoet ratio and dose?
I got this from fitting a straight line to Table 1 of Banu's 2004 paper:
10.1107/S0907444904007917
Is this a rough estimate based on a singular result? Of course it is!
This
Thank you to all who provided helpful suggestions so far.
A few things I'd recommend for this particular beamline (which I have
been running for 20+ years)
Do NOT collect one wavelength at a time. This was a good strategy on old
beamlines with noisy detectors and slow, drifty monochromators.
CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection
etc. tips
Hi
With this in mind, I have found the easiest way to generate AlphaFold models
(for those not in the DB) is actually in CCP4 Cloud-remote - makes MR using AF
models a real doddle.
See the Tut
From: CCP4 bulletin board on behalf of dbellini
Sent: Tuesday, May 14, 2024 06:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Experimental phasing Selenomethionine data collection
etc. tips
[You don't often get email from dbell...@mrc-lmb.cam.ac.uk. Learn
Hi
With this in mind, I have found the easiest way to generate AlphaFold models
(for those not in the DB) is actually in CCP4 Cloud-remote - makes MR using AF
models a real doddle.
See the Tutorials (in particular on MR) available with CCP4 Cloud.
Harry
> On 14 May 2024, at 09:20, Randy
Dear Marco,
You don’t mention here or in the earlier thread whether you tried AlphaFold
models and, if you did, how you prepared them for MR. I’m happy to hear of any
case where we still need experimental phasing methods to solve new protein
structures, but we’ve seen very few examples where
Hi Marco,
A few suggestions that I like to follow for MAD experiments:
Before everything, check you have at least about 1 SeMet per 100
residues
Then before crystallisation check by MassSpec that SeMet is properly
incorporated in your protein
After crystallisation collect first on the peak
Hello all,
I have a data collection trip next week and plan to collect data on
selenomethionine derivative crystals at the al831 beamline. Are there any
resources, tips, tutorials, literature etc. That you can recommend to help me
prepare for these experiments. Also is there a way to plug in
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