Dear all,
I have 3 short questions about PEG solutions:
Does anyone know the best way to store crystallization screening blocks that
contain PEG 3350?
Is it a good idea to freeze the PEG solutions at -80°C and thaw them before use?
Would the freeze-thaw process considerably alter the PEG chain
Hi Jerome,
-I have heard that PEG solutions can become unstable in light. We usually
store our block in the fridge, where photons are scant anyway. For any
stocks that I prepare, I wrap the tube/bottle in aluminum foil. I'm not
sure about freezing them.
-Some labs (not ours) evidently prepare
I don't think storage matters. I doubt Hampton stores their PEG stock
solutions at -80 before they ship out to customers.
I've solved tons of structures leaving my PEGS and PEG screens at RT in the
light.
Nick
On Mon, Jul 14, 2014 at 12:32 PM, Chris Fage cdf...@gmail.com wrote:
Hi Jerome,
Jerome,
Does anyone know the best way to store crystallization
screening blocks that contain PEG 3350?
I would recommend storing them in a fridge or a clean coldroom (mold-free).
Lower temperature and low light does help.
Is it a good idea to freeze the PEG solutions
at -80°C and thaw
I also used to store my PEG solutions in light, and my stocks do sit
out on the bench. I can't say for sure whether light or temperature
make a difference, but I like to heed what seem like superstitions in
crystallography to eliminate variables. We purchase our screens from
Qiagen, who suggests
Michael, Chris and Nick,
Thank you so much for your help.
-Jerome
Jerome Nwachukwu
jnwac...@scripps.edumailto:jnwac...@scripps.edu
On Jul 14, 2014, at 1:48 PM, R. M. Garavito
rmgarav...@gmail.commailto:rmgarav...@gmail.com wrote:
Jerome,
Does anyone know the best way to store
