Vincent,
You are on the right track. Notice now that both maps at chi/kappa=180 have
strong peaks at the y-axis and a “mirror” plane perpendicular to this.This
is what would be expected for P21, i.e., Y is b/b*. Then depending on the
orthogonalization convention of MolRep, the a- and
this explains things a little,
Best, Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von vincent
Chaptal
Gesendet: Freitag, 2. Juni 2017 12:05
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] help needed to interpret a SRF
Hi Michael,
thank you for your explanations.
On 01/06
Vincent,I see a few of problems with your SRF (the maps) which would impact the interpretation. First you say that both crystals are processed as P21, which you would expect a very strong peak (100% of your origin peak) on kappa/chi=180 at the b* axis (one of the major axes of your map); this
Thank you for your email.
the anisotropic resolutions of the datasets are 5.6-7.1A for the best
and worst diffracting directions of the crystal without additive, and
4.0-5.8A for the crystal with additive.
The two crystals come from the same prep and same drop setup, only
differ from the
Well - you dont give details o f resolution but the sovent content and the
peak of 0.51 would suggest a possible dimer in crystal 1
Crystal 2 is so different it might well have a dimer in a different
orientation.
I would try to see if there was a relationship between Xtal 1 and Xtal 2 -
can
Dear all,
I need help interpreting results from a SRF; I am very naïve at
interpreting them and would appreciate any pointer...
I have 2 crystals, before and after additive during crystallogenesis.
They have different cell parameters, and I am wondering if I have a
monomer or a dimer in the
