In theory, what you say is quite sensible. But there is one interesting
counter example I am aware of.The fragment tool compound that
eventually gave rise to the clinical compound indeglitazar (
http://www.pnas.org/content/106/1/262.full.pdf) gives a negative shift by
DSF (in our hands):
ubject: [ccp4bb] off-topic: negative thermal shift upon ligand binding
To: ccp4bb@jiscmail.ac.uk
Hello,
I am working on DSF to verify if some compounds bind to my protein. I see a
negative shift of about 3-4 degrees upon ligand addition (dose-response) in
comparison to the protein alone. I
Hello,
I am working on DSF to verify if some compounds bind to my protein. I see a
negative shift of about 3-4 degrees upon ligand addition (dose-response) in
comparison to the protein alone. I assume that this might be due to the
binding of compound to the unfolded stated rather than folded
