Re: [ccp4bb] problem in heavy metal soaking

2010-09-27 Thread vincent Chaptal
...@saha.ac.in Subject: [ccp4bb] problem in heavy metal soaking To: CCP4BB@JISCMAIL.AC.UK Date: Sunday, September 26, 2010, 9:00 AM I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M AmSO4,my question is if there's any problem if I want to soak heavy metal derivatives

[ccp4bb] problem in heavy metal soaking

2010-09-26 Thread Seema Nath
I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M AmSO4,my question is if there's any problem if I want to soak heavy metal derivatives in this crystallizing condition? Does AmSO4 interfere in heavy-metal soaking ? if yes, what's the reason? Thank you in advance.

Re: [ccp4bb] problem in heavy metal soaking

2010-09-26 Thread Patrick Loll
On 26 Sep 2010, at 9:00 AM, Seema Nath wrote: I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M AmSO4,my question is if there's any problem if I want to soak heavy metal derivatives in this crystallizing condition? Does AmSO4 interfere in heavy-metal soaking ?

Re: [ccp4bb] problem in heavy metal soaking

2010-09-26 Thread Paul Smith
Nath seema.n...@saha.ac.in Subject: [ccp4bb] problem in heavy metal soaking To: CCP4BB@JISCMAIL.AC.UK Date: Sunday, September 26, 2010, 9:00 AM I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M AmSO4,my question is if there's any problem if I want to soak heavy metal