In addition to all the excellent suggestions to perform seeding, you could also
try sitting drop vapor diffusion, and also different temperatures. Sometimes
moving from 20 to 4 degrees does the trick.
Diana
**
Diana R. Tomchick
Professor
Hi Nikolaus
I completely agree with Claude's comments. Microseeding is the first thing
to try. You would like to find conditions where you *don't *get crystals
without seeding, but you *do *get crystals with seeding. Then, just by
diluting the seed stock, you can control nucleation. Look at
Le 09/09/2019 à 17:22, Nikolas a
écrit :
Dear
crystalgrowers,
I am
currently working with a protein that appeared to be
friendly but turned out it was not the case.
I
Hi Nikolas
The first thing I would try is to solubilise the protein a bit more, maybe by
adding a few % (1-5%) glycerol or ethylene glycol to the growth conditions when
you set up the drops.
The next thing would be to experiment with ratio (protein:precipitant) and drop
size.
Lastly, trial
