Re: [ccp4bb] suggestions are welcome

2017-07-19 Thread Francesca Malvezzi
Hi, often co-expressing and purifying proteins can help complex formation. Best, Francesca On Tuesday, July 18, 2017 4:18 AM, El Sahili Abbas (Dr) wrote: Hi, This might indicate that the binding affinity of your complex is not high enough for it to resist the

Re: [ccp4bb] suggestions are welcome

2017-07-17 Thread El Sahili Abbas (Dr)
Hi, This might indicate that the binding affinity of your complex is not high enough for it to resist the gel filtration dilution. Maybe running a native PAGE gel would allow you to visualise a complex. Best A > On 18 Jul 2017, at 10:25, 高艺娜 wrote: > > Hi all , > > It has