This is a very educational thread but I should remind you that the
assumed distributions are NOT reliable when either a) the data is very
anisotropic, or b) the data is very incomplete or c) there is a
non-crystallographic translation vector in the structure or d) the data
is twinned.
I for
Hey there,
I am working on (the theoretical side of) a protein complex whose
structure has been solved. The protein homo-dimerizes, mediated
primarily by two long helices.
Using sequencing alignment and the WHAT IF server, I built monomeric
hybrid models containing the bulk of the known
Dear All,
a very late reaction, but we have a Dell proyector that elicit the
same reaction as yours did.
In another building there is a BenQ MP622c, which is much, much better.
Mark
This is way off-topic, but that's never stopped me before. And what
group is better qualified to
Institute for Biochemistry, University of Greifswald, Germany
Dear All,
In the group of Prof. Hinrichs a postdoctoral position is available
for initially 3 years. The position involves research and teaching,
usually also for undergraduates. Some knowledge in German is thus
preferred, you
-Original Message-
From: Bart Hazes [mailto:[EMAIL PROTECTED]
Sent: 08 September 2008 23:44
To: Ian Tickle
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] truncate ignorance
How a seemingly innocent question can explode ...
Well there seems to be a widespread misunderstanding
For comparison I repeated my previous calculation using the flat
(non-Wilson) prior that you suggested (you would get the same results
using the FW method in the limit of an infinite WDP). So now the
results are indeed totally independent of the WDP - but IMO the results
are also completely
Thanks for all the interesting answers so far!
The anisotropy issue is one that got me worrying about
truncate for data from DNA-containing crystals in
particular - and the fact that since its a default in ccp4i,
new people have stopped worrying about whether or not they
should use it.
The
On Sep 9, 2008, at 8:12 AM, Phoebe Rice wrote:
Thanks for all the interesting answers so far!
The anisotropy issue is one that got me worrying about
truncate for data from DNA-containing crystals in
particular - and the fact that since its a default in ccp4i,
new people have stopped worrying
AW: [ccp4bb] truncate ignoranceI have had a general crystallogrphy question for
quite a while, upon which perhaps the CCP4BB members can shed some light: Are
small shifts in cell parameters real?
I know that datasets at cryo vs. rt, for example, are often the same
spacegroup, but have smaller
How would you tell the difference between a unit cell shift and a wavelength
shift when collecting diffraction data at a synchrotron beamline? Well, all
the cell length would scale by the wavelength, so that would be one hint
that the wavelength changed. If a got longer and c got shorter, then
AW: [ccp4bb] truncate ignoranceIn thinking about one or two responses, I am
wondering whether mosaicity could be the result of having a distribution of
each of the cell parameters within one crystal? Is that what mosaicity really
is? That is, if axis a has a broad distribution of lengths, due
lurk mode
The answer I think is yes, but partially. Colin Nave published a very
nice paper on this, A description of Imperfections in Protein Crystals
in Acta Cryst D54, 848-853 (1998). The 'real' mosaicity is a
convolution of the variation in cell dimensions within crystallites or
domains
Dear CCP4BBs,
I post the question for one of the staff in our lab:
Hi everyone
Does anyone have the experience of using Phoenix robot automatically setup the
crystal? Which plate the Phoenix could use such as 96-well sitting drop or
hanging drop? If available could you also tell me the CAT NO.
Andreas,
Here's my $0.02. Would you mind clarifying a few things for me?
I am working on (the theoretical side of) a protein complex whose
structure has been solved. The protein homo-dimerizes, mediated
primarily by two long helices.
So you have a structure of a homodimer...
Using
Dear Hao --
On 10 Sep 2008, at 02:00, jxqi wrote:
Does anyone have the experience of using Phoenix robot
automatically setup the
crystal?
yes
Which plate the Phoenix could use such as 96-well sitting drop or
hanging drop?
96 sitting-drop
If available could you also tell me the CAT NO.
Does anyone have the experience of using Phoenix robot automatically setup thecrystal? We have had a Phoenix in our lab for about 2 years now, and have been very pleased with the performance. Other users I spoke with have also expressed the same satisfaction.Which plate the Phoenix could use such
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