I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M
AmSO4,my question is if there's any problem if I want to soak heavy metal
derivatives in this crystallizing condition? Does AmSO4 interfere in
heavy-metal soaking ? if yes, what's the reason?
Thank you in advance.
On 26 Sep 2010, at 9:00 AM, Seema Nath wrote:
I'm working with a protein which crystallizes in a mixture of PEG6K with 0.2M
AmSO4,my question is if there's any problem if I want to soak heavy metal
derivatives in this crystallizing condition? Does AmSO4 interfere in
heavy-metal soaking ?
test of subscription change
I am working on 45 kd protein (pI 5.3) for crystallization . It is in pGEX
vector.I do the expression (induction 24 degree 16 hrs) and purification from
Rosetta 2 DE3 cells (has additional rare codon).when I run the affinity
chromatography purified protein (cleaved protein after removal of
What are the minor differences in peptides ?
Any PTM's perhaps ?
Jürgen
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
I assume that the loss of the peptides that you observe for the smaller protein
is at the other terminus from the tag? If it is at the terminus where the tag
was it would suggest that removal of the tag using proteolysis is the most
likely cause. Though saying that Factor Xa/thrombin is not
I'm interested in opinions/advice on home source systems.
As synchrotron beamlines are more powerful and more accessible than ever, a
home source is really only needed for crystal screening, if at all.
With that idea in mind, what are options out there for buying and running a
home source in
Hi Seema,
In theory, Ammonium Sulfate contains some small fraction of neutral ammonia
which can act as a strong nucleophile and react with many heavy metal compounds.
That being said, I recently phased two structures with mercury soaks, both of
which contained ammonium sulfate. The first was
1. your information is helpful but not enough to tell if this double-band
pattern is a product of e.g. proteolysis or of abortive translation. You
don't specify what these 'minor differences' are - in the world of MS there
is no such thing as 'minor' since every difference is either a significant
