Re: [ccp4bb] Difficult experimental phasing

Estimating isomorphism by eyeballing the difference in unit cell lengths may mask significant non-isomorphism that is present. Iodine is a large atom and it is notoriously difficult to get an iodine derivative that is isomorphous to a native protein. Depending upon the chemistry of your mother

[ccp4bb] Acrimboldo BORGES standard libraries

Hi all, I have been trying to install the BORGES standard libraries to run ACRIMBOLDO with them. This is an installation of the libraries on top of a CCP4 installation using 'ccp4sm'. The libraries download, the installation finishes and the libraries end up in the following folder:

[ccp4bb] Difficult experimental phasing

Dear all, I’m trying to solve the structure of a putative homodimer, but despite having collected good quality data, it has proven to be rather difficult. I have collected native and derivative datasets (2.2-4Å, C2 SG, 165/35/115Å//104° unit cell) with acceptable merging statistics. Xtriage,

Re: [ccp4bb] Suggestions on Dynamic Light Scattering instruments

Dear Chandramohan, We use Malvern particle size analyzer (ZEN 3690 ZETASIZER NANOZS 90, version 7.03) fitted with 21CFR part 11 software. It works fine for us in terms of measuring soluble proteins particles in size range from nanometre to micrometre. Hope this helps. Cheers!! Debasish

[ccp4bb] Suggestions on Dynamic Light Scattering instruments

Dear all, We are looking for Dynamic Light Scattering instruments to test our purified protein complexes prior to crystallization. Any suggestions or recommendations regarding which instruments to pursue? Thanks in advance Chandra kattamuricha...@yahoo.com

Re: [ccp4bb] size exclusion columns

Dear Markus Please find a response from one of our specialists - feel free to contact him for more information (his contact details are at the bottom of this email as he is not on the bulletin board). The primary factors I would consider are the type of material in the column and also the

[ccp4bb] Paul Scherrer Institut (PSI) :: Scientist

https://www.psi.ch/pa/job-opportunities/1753 Prof. Gebhard F.X. Schertler Structural Biology ETH Zürich D-BIOL Head of Biology and Chemistry Division Paul Scherrer Institut Laboratory of Biomolecular Research, LBR OFLC 109 CH-5232 Villigen PSI

[ccp4bb] Two Postdoctoral Research Posts in Oxford

https://www.path.ox.ac.uk/content/postdoctoral-research-assistant-structural-biologyprotein-chemistry-2-posts POSTDOCTORAL RESEARCH ASSISTANT IN STRUCTURAL BIOLOGY/PROTEIN CHEMISTRY (2 POSTS) Closing date: Friday, 11 May 2018 - 12pm Grade 7: £31,604 - £38,833 p.a. We are seeking to appoint two

Re: [ccp4bb] Calculating sigma value

Dale, On 19 April 2018 at 17:36, Dale Tronrud wrote: >The meaning of the term "e/A^3" as used in Coot has nothing to do > with the charge of an electron. The intention of its authors is to > indicate that the value being represented by the map is the density of >

Re: [ccp4bb] determining the point group and the space group

Hi Gihan, and anyone else who finds this interesting. Sorry for the late response: > how should one determine a point group and the space group of an > unknown crystal? > > I have a protein crystal with know unit-cell parameters. (these are > XFEL data so indexing wouldn't give the point and