[ccp4bb] Glutathione rsin

Hi, I would like to ask the bb faithful for their experience with the glutathione affinity resins. We have been using so far the Glutathione Sepharose fast flow from GE but the price is getting steeper. We found Glutathione Superflow resin from Clontech to be significantly less expensive and

[ccp4bb] OSX 10.9.2

Hi, Does the ccp4 suite work with the latest OSX 10.9.2 operating system? Are there any known problems? Mirek

[ccp4bb] PyMol and Schrodinger

Hi, I have inquired at Schrodinger about the licensing for PyMol. I was surprised by their answer. The access to PyMol is only through a yearly licence. They do not offer the option of purchasing the software and using the obtained version without time limitation. This policy is very different

[ccp4bb] shelxCDE in ccp4i

Hi, I have shelx programs installed on my computer but only shelxs shows as active on the ccp4i interface. How to activate shelxCDE? Thanks, Mirek

[ccp4bb] Tenure Track position in Structural Biology at the University of Saskatchewan

Tenure Track Position in the College of Medicine at the University of Saskatchewan The College of Medicine invites applications for a tenure track faculty position at the Assistant, Associate or Full Professor level in the area of structural biology of membrane proteins, or in the application

[ccp4bb] OS X yosemite

Hi, I am thinking of upgrading the os on my mac to Yosemite. Are there any known issues for crystallographic software that I should pay attention to? Thanks, Mirek

[ccp4bb] XQuartz problems

Hello Mac Gurus, I have run today into a problem with XQuartz on my iMac. No problems till now. Now, any application that uses X11 does not start. It invokes XQuartz but that is it. What is more, when running, XQuartz can not be stopped; after an attempt to quit the application XQuartz

Re: [ccp4bb] Problem with Mg2+ binding site refinement

Sent from my Bell Samsung device. Original message From: Clemens Vonrhein Date: 2017-06-16 4:43 AM (GMT-06:00) To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Problem with Mg2+ binding site refinement Hi, On Fri, Jun 16, 2017 at 10:47:20AM

[ccp4bb] gif dictionary for connecting sugars

Hi, I have difficulty with the cif dictionary to connect sugars in a tetrasaccharide. The dictionary works well in coot (regularizes as expected) but when refined in Phenix, one connection misbehaves. Where can I find description of the rules for creating the link? Which residue is taken as '1'

[ccp4bb] PyMol question

Hi, When I loaded the ed map into PyMol v1.8.4.1 and used iso mesh around part of the protein all is well until I use the RAY command. In the ray traced image I see the unit cell box that does not show on the image in the normal view. How can I remove the box from the ray traced image? Thanks

[ccp4bb] Assistant Professor positions at the U of Saskatchewan, Canada

Dear Colleagues, Our department is recruiting two new faculty. The structural biology is among the research areas that are considered. I would be grateful if you could bring the attached ad to the attention of qualified candidates. Many thanks and best wishes, Mirek

[ccp4bb] Coot labels

Hi, I just started using coot 0.9 and see some differences in behaviour from version 0.8. When I move to the next residue using space bar the residue name of the residue does not appear. How can I make appear automatically? Best, Mirek

[ccp4bb] Comparing two datasets

Hi, I would like to calculate the R-merge for Fs from two datasets processed from two different crystals. Tried to use Blend but got the message that Blend requires R. Downloaded R but do not know how to tell CCP4 where it is located on my Mac. Is there another program that would take two mtg

Re: [ccp4bb] Comparing two datasets

XSCALE will do that for you : https://xds.mr.mpg.de/html_doc/xscale_program.html If not, maybe have a look on SHELXC in SIR mode. Hope this help. Nicolas On 25/07/2022 21:52, Cygler, Miroslaw wrote: Hi, I would like to calculate the R-merge for Fs from two datasets processed from two different

[ccp4bb] Missing file

When I start coot on Mac there are several warnings: /Applications/ccp4-8.0/coot_py2/bin/coot: line 81: /Applications/ccp4-8.0/coot_py2/bin/ccp4.setup-sh: No such file or directory WARNING:: Coot REFMAC dictionary override COOT_REFMAC_LIB_DIRfailed to find a dictionary

[ccp4bb] Coot issue

Hi, I just upgrade my Mac to Ventura v13.2 from v12.6.3 and run into issue with Coot. When I use the delete command it causes the main Coot window disappearing while other Coot windows stay open. Has anyone had this issue? Is there a way around it? No such problem on v12.6.3. Best, Mirek

[ccp4bb] Tenure track position in Protein Science for Pathogen Defense

Dear Colleagues, I am excited to let you know that the Department of Biochemistry, Microbiology and Immunology, College of Medicine, University of Saskatchewan, is searching for an exceptional candidate in the area of Protein Science for Pathogen Defense, to fill a tenure-track Canada Research