Re: [ccp4bb] Color of heme containing Xtals

2008-03-12 Thread R Michael Garavito
better, more information is needed. Cheers, Michael * R. Michael Garavito, Ph.D. Professor of Biochemistry Michigan State University East Lansing, MI 48824-1319 517-355-9724 (Office) 517-353-9334 (Fax) [EMAIL PROTECTED

Re: [ccp4bb] Protein Purification

2017-01-18 Thread R. Michael Garavito
R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: rmg

Re: [ccp4bb] Density for an unknown ligand

2016-11-29 Thread R. Michael Garavito
R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-

Re: [ccp4bb] Help needed finding hit condition

2017-07-31 Thread R. Michael Garavito
) was pH 8, but when diluted down with the other components to make the 150 mM concentration for the screen mixture, the pH can be significantly different. The dilution of Tris would drop the pH. Cheers, Michael R. Michael

Re: [ccp4bb] mystery disappearance of CCP4 website(s)

2017-05-25 Thread R. Michael Garavito
R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: rmgarav...@gmail.com

Re: [ccp4bb] mystery disappearance of CCP4 website(s)

2017-05-25 Thread R. Michael Garavito
I checked again after Peter’s email and they are reachable. WTF May they did pay, as Bernhard suggests. Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan S

Re: [ccp4bb] help needed to interpret a SRF

2017-06-02 Thread R. Michael Garavito
xis and the second peak in the X-Z plane. These could be weak NCS two-folds. How many NCS systems do you suspect in the ASU? Cheers and good luck, Michael ************ R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biol

Re: [ccp4bb] Can DDM give egregious false positives when using it to crystallize membrane proteins?

2017-06-16 Thread R. Michael Garavito
chael **** R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334

Re: [ccp4bb] help needed to interpret a SRF

2017-06-01 Thread R. Michael Garavito
xis (North-South or y-axis).Cheers,Michael EhaAc_kappa180.pdf Description: Adobe PDF document ********R. Michael Garavito, Ph.D.Professor of Biochemistry & Molecular Biology603 Wilson Rd., Rm. 513   Michigan State University      East Lan

Re: [ccp4bb] ccp4 website not secure

2017-10-11 Thread R. Michael Garavito
/ <https://www.ccp4.ac.uk/ccp4online/>, which requires logins, but ccp4 is http://www.ccp4.ac.uk/ <http://www.ccp4.ac.uk/> still. I had it mostly when connecting from home. Michael ******** R. Michael Garavito, Ph.

Re: [ccp4bb] Differences in a homodimer protein

2017-11-29 Thread R. Michael Garavito
. Bottom line is to be cautious and skeptical with any hypothesis you want to test about this water. Cheers, Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan S

Re: [ccp4bb] A helix with leucine repeats

2018-03-03 Thread R. Michael Garavito
, Michael R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 513 Biochemistry Bldg. Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-