.
www.oxford-diffraction.com
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: 26 January 2010 16:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo
.
www.oxford-diffraction.com
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: 26 January 2010 16:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo conditions or the
crystals
A : CCP4BB@JISCMAIL.AC.UK
Copie à :
Objet : Re: [ccp4bb] Crystal rescue
Thanks for so many quick responses!
Actually, I have test several different cryo-protectants, including
glycerol, EG, and PEG400. I did not see much differences between these
cryo conditions. So, I choose glycerol.
I would like
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: 26 January 2010 16:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo conditions or the
crystals. Depending where you
RT system very useful.
Good luck!
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: Wednesday, January 27, 2010 7:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
MiTeGen (www.mitegen.com) sells a RT device
, 2010 7:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
MiTeGen (www.mitegen.com) sells a RT device - plastic capillary to put
over loop... I do not know how well it would work for a long data
collection - but people here have used it to evaluate their crystals
Ezra
On 1/27
Hi Zhiyi,
I think the easiest way is to mount your crystal in an
air-impermeable, viscous oil like Paratone in a loop. Of course, your
crystals may not tolerate oil.
ho
First you need to establish if it is your cryo conditions or the
crystals. Depending where you are - they might have the equipment to do
a wet mount - without freezing. Yes the crystal will not last - but
then you know if the problem is in the
crystal. If it is - you need better crystals.
http://hamptonresearch.com/documents/product/hr000175_what_is_tacsimate_new.pdf
turns up once you use google's I'm feeling lucky button.
On Tue, 2010-01-26 at 15:42 +, Zhiyi Wei wrote:
Dear all,
I got a problem with my crystals. I have two total different proteins
that both can be
I forgot to mention a phenomenon when I did crystal mounting. I found
that if open a coverslip, phase separation will appear in drops in
minutes and damage to the crystals.
And I have tried additive screen. The optimized crystals are really
big (a few hundred microns) with sharp edge but
how much %PEG3350 ?
Try different additives such as glycerol, EG, MPD or smaller PEGs 200
or 400.
Try flash annealing
Try freezing directly in liquid nitrogen if you have previously frozen
your crystals in the stream
Try liquid annealing by dipping the frozen crystal into a new cryo
,
Marcus Winter.
www.oxford-diffraction.com
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: 26 January 2010 16:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo conditions or the
crystals. Depending where you are - they might have the equipment to do
a wet mount - without freezing. Yes the crystal will not last - but
then you know if the problem is in the
crystal
Hi, Zhiyi:
You can always put a layer of oil on the top of your drop when you
open the coverslip. It will get you more time to mount the crystals.
Good luck
Meng-Chiao Ho
On Tue, 2010-01-26 at 11:48 -0500, Zhiyi Wei wrote:
I forgot to mention a phenomenon when I did crystal mounting. I found
-diffraction.com
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Ezra Peisach
Sent: 26 January 2010 16:01
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal rescue
First you need to establish if it is your cryo conditions or the
crystals
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