Re: [ccp4bb] How to make GDP.BeF3 solution ?

Dear Firdous, As Matt refers BeF3 mimics the GTP/ATP bound states. For the transition state we have used mixtures of AlCl3 and HKF2 in combination with GDP (or ADP) and MgCl2 within the buffer. Be aware that depending on the concentrations used of each component you get different proportions

Re: [ccp4bb] How to make GDP.BeF3 solution ?

MAIL.AC.UK Subject: Re: [ccp4bb] How to make GDP.BeF3 solution ? Be aware that beryllium is also quite toxic. https://en.wikipedia.org/wiki/Acute_beryllium_poisoning Diana ** Diana R. Tomchick Professor Departments of Biophysics a

Re: [ccp4bb] How to make GDP.BeF3 solution ?

Hi Firdous Can you ask Trilink biotechnologies if they can make it for you very good experience many speciality materials made Disclaim: i do not work with them or have any financial connections with them I think jena discontinued this item

Re: [ccp4bb] How to make GDP.BeF3 solution ?

@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How to make GDP.BeF3 solution ? Dear Firdous, beryllium fluoride is actually a ground state analogue of GTP as trifluoroberyllate is tetrahedral. To get a transition sate analogue you need either AlF4- or MgF3-. Preparation of these complexes is very easy

Re: [ccp4bb] How to make GDP.BeF3 solution ?

] How to make GDP.BeF3 solution ? Dear Firdous, beryllium fluoride is actually a ground state analogue of GTP as trifluoroberyllate is tetrahedral. To get a transition sate analogue you need either AlF4- or MgF3-. Preparation of these complexes is very easy. The great advantage of metal

Re: [ccp4bb] How to make GDP.BeF3 solution ?

Dear Firdous, beryllium fluoride is actually a ground state analogue of GTP as trifluoroberyllate is tetrahedral. To get a transition sate analogue you need either AlF4- or MgF3-. Preparation of these complexes is very easy. The great advantage of metal fluoride transition state analogue and

[ccp4bb] How to make GDP.BeF3 solution ?

Hi I would appreciate it if someone could share with me a step by step protocol for making a stable GDP.BeF3 solution which is often used as a transition state analogue for structural studies of a protein complex ? Or a vendor from where it can be purchased directly. Regards Firdous