Re: [ccp4bb] the structures of Nucleic acid

2023 at 4:44 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] the structures of Nucleic acid I am afraid most scientists will use the most straightforward technique! If SAD is available the PHOSPHATE backbone of DNA will provide sufficient signal to allow SAD to work, and you get an unambigu

Re: [ccp4bb] the structures of Nucleic acid

nar/gkaa439) and >> Ca could be attractive as a potential anomalous scatter as well. >> >> Best regards, >> >> Armin >> >> >> >> >> From: CCP4 bulletin board on behalf of Mark J. van >> Raaij >> Date: Tuesday, 1

Re: [ccp4bb] the structures of Nucleic acid

ential anomalous scatter as well. > > Best regards, > > Armin > > > > > From: CCP4 bulletin board on behalf of Mark J. van > Raaij > Date: Tuesday, 19 September 2023 at 12:31 > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] the structures of Nu

Re: [ccp4bb] the structures of Nucleic acid

aaij mailto:mjvanra...@cnb.csic.es>> Date: Tuesday, 19 September 2023 at 12:31 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] the structures of Nucleic acid This just appeared and may be relevant: https://academic.oup.c

Re: [ccp4bb] the structures of Nucleic acid

on behalf of Mark J. van Raaij Date: Tuesday, 19 September 2023 at 12:31 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] the structures of Nucleic acid This just appeared and may be relevant: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad726/7272628 Critical Reviews and Perspectives

Re: [ccp4bb] the structures of Nucleic acid

This just appeared and may be relevant: https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad726/7272628 Critical Reviews and Perspectives When will RNA get its AlphaFold moment? Mark van Raaij Dpto de Estructura de Macromoleculas, lab 20B Centro Nacional de Biotecnologia - CSIC

Re: [ccp4bb] the structures of Nucleic acid

The phosphorus absorption edge is about 5.8Å. I've had much better luck with 5-Br-U for anomalous phasing. Molecular replacement with sub-structural fragments can also work: Yours sincerely, William G. Scott Professor,

Re: [ccp4bb] the structures of Nucleic acid

I am afraid most scientists will use the most straightforward technique! If SAD is available the PHOSPHATE backbone of DNA will provide sufficient signal to allow SAD to work, and you get an unambiguous answer to whether it is A-DNA or B or Z... MR will usually work of course as well Eleanor On

Re: [ccp4bb] the structures of Nucleic acid

Dear Fu Xingke, Depends on what Nucleic Acid you are talking of. If it is RNA, you can expect some sequence to tertiary structure correspondence so you might be able to try more MR as compared to DNA. DNA may have double helical architecture but less sequence to tertiary structure

[ccp4bb] the structures of Nucleic acid

Dear Colleagues, Reacently, I find the structures of Nucleic acid are solved by single-wavelength anomalous diffraction(SAD). So, why molecular replacement (MR) not? Regards Best wishes, Fu Xingke Institute of Physics CAS