Hi Gaoyina,
Maybe a stupid suggestion, but did the paper of the negative stain EM explain
how the complex was prepared? That would be the first thing I would try.
Further, as Abbas explained, the complex may fall apart during gelfiltration.
At least for analytical purposes, you could try to
I am very happy that our colleagues give a lot of valuable suggestions.
Sometimes you need to select a good example to teach students. In this regard
5XQL seems a really unbelievable paradigm--poor data, rookie crystallographer,
challenging molecular replacement and overnight accepted
Postdoctoral Fellow Position Parashar laboratory at Rutgers School of Dental
Medicine iscurrently seeking a highly motivated, creative individual with
strong interestin the structure-function studies of proteins regulating
bacterial signaling. Qualifications:
- Ph.D. in Biochemistry,
Dear Grace,
There are two ways that you can do this. If you run
POINTLESS/AIMLESS from iMosflm using the “Quickscale” option, you can tell
POINTLESS to use the symmetry set at the indexing stage, using SETTINGS ->
Processing options -> Sort Scale and Merge tab,, select the
