[ccp4bb] Position available

Dear all: Please see the advert below regarding an exciting position which is available at the Albert Einstein College of Medicine in New York City: The laboratory of Tyler Grove and Steven Almo at the Albert Einstein College of Medicine seeks a highly motivated and innovative post-doctoral

Re: [ccp4bb] weird diffraction pattern

Hi, Thanks to all who gave me suggestions concerning the weird diffraction pattern and I really appreciate it that Kay Diederichs help me processing my data set and answer my questions. Although the data set can be processed using HKL3000, XDS without problems, the Rwork/Rfree values are still

Re: [ccp4bb] weird diffraction pattern

Hi Chenjun Tang, I have not followed the original discussion, so my apologies if I am repeating the device already given to you. Crystallization optimization is always a good idea - nothing beats good quality crystals. In addition, you should try collecting few images, especially in the

Re: [ccp4bb] Coot no GUI

coot --no-graphics William G. Scott http://scottlab.ucsc.edu > On Jul 28, 2017, at 1:34 PM, Reza Khayat wrote: > > Hi, > > Is it possible to run Coot without the use of GUI? We have some Coot scripts > for a pipeline and we’d like to do without the GUI. Thanks. >

Re: [ccp4bb] Best method for carbohydrate refinement

Hi Gustavo, If I understand you correctly, you are concerned about N-glycans (N-glycosylation) on your proteins. According to your description, you have 2 protein molecules in each ASU, each bearing one potential N-glycan site. Then there are only two N-glycan sites you need to build for

[ccp4bb] Coot no GUI

Hi, Is it possible to run Coot without the use of GUI? We have some Coot scripts for a pipeline and we'd like to do without the GUI. Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor Department of Chemistry City College of New York 85 Saint Nicholas Terrace, CDI 2.318 New York,

[ccp4bb] Best method for carbohydrate refinement

Hello everybody, I was looking for suggestion on the best way to identify and refine carbohydrates in a protein. This is the scenario: - I have 10 molecules in the biological assembly - I used sf9 expression system, so I have random glycolisation and types of glycolisations. I have some hint

Re: [ccp4bb] Best method for carbohydrate refinement

Depending on your resolution or the quality of the local sugar density you also may want to give the GLYCAM server by Rob Woods' group a try. It includes a straightforward glycan builder, so no format issues, and does a great job in finding the lowest energy conformation for complex carbs,