Is there any additives in your buffer which can absorbing at 280nm ?
Satya
On Tue, Feb 10, 2015 at 1:31 PM, Keller, Jacob kell...@janelia.hhmi.org
wrote:
Or use copper staining—just add 300 mM copper chloride to your gels
right after running, see your results in ~5 min, when non-protein
Hello Jaimohan,
Is it SDS-PAGE gel or Native PAGE gel ?. Theoretically SDS-PAGE is
supposed to show monomers and Native PAGE oligomers
On Tue, Jun 27, 2017 at 8:27 AM jai mohan <
0cab66323371-dmarc-requ...@jiscmail.ac.uk> wrote:
> Dear all,
> I am working on a Red Fluorescent
, which you are going to solve the crystal structure.
This is another way of obtaining better crystals in addition to above
mentioned useful comments.
Wish you good luck for better crystals,
Satya
On Wed, Feb 14, 2018 at 12:33 PM, Kevin Jin <kevin...@gmail.com> wrote:
> Hi Yu,
>
>
are
as follows
Mean I / sig I : Overall: 11.3 OuterShell: 5.0 InnerShell: 20.0
CC(1/2) : Overall: 0.995 OuterShell: 0.942 InnerShell: 0.997
Thank You,
Satya Dev.
On Fri, Mar 17, 2017 at 7:59 PM, Mohamed Noor <mohamed.n...@ul.ie> wrote:
> How do you know it's really 2 A resolu
Hi,
We checked all possible space groups of orthorhombic crystal system using
Scala and Pointless but the statistics show that P212121 is the possible
space group.
Thank You,
Satya Dev
On Fri, Mar 17, 2017 at 8:03 PM, Teplyakov, Alexey [JRDUS] <
atepl...@its.jnj.com> wrote:
> Check
-- Forwarded message --
From: Polisetty Satya Dev <pvss...@gmail.com>
Date: Mon, Mar 20, 2017 at 1:35 PM
Subject: Re: [ccp4bb] No improvement in R-factor after Refmac.
To: Sudipta Bhattacharyya <sudiptabhattacharyya.iit...@gmail.com>
Hi,
I had tried running xtriage
, 156.96, 90.00, 90.00, 90.00,
Space group: P212121,
Completeness 99.5,
Multiplicity 6.4,
Four monomers per ASU.
Solvent content: 47%.
Thank you everyone,
Satya Dev,
JNCASR.
