Hello Jaimohan, Is it SDS-PAGE gel or Native PAGE gel ?. Theoretically SDS-PAGE is supposed to show monomers and Native PAGE oligomers........
On Tue, Jun 27, 2017 at 8:27 AM jai mohan < 00000cab66323371-dmarc-requ...@jiscmail.ac.uk> wrote: > Dear all, > I am working on a Red Fluorescent Protein (His-Tag) molecular weight > around 27kDa. After purification I ran a SDS page, the band at 27kDa > confirms the monomer. The protein was stored at -20C, a week later again I > ran a gel, this time I saw another new band between 50-60kDa, it confirms > the protein solution contains both monomers and dimers. I would like to > know, what is the best way to separate the monomers and dimers? One of my > colleague advice me to go for sucrose gradient centrifugation and size > exclusion chromatography. > However, I seek all your valuable suggestions and advice. > > With best regards > Dr. S.M.Jaimohan >
