Re: [ccp4bb] Diffraction image viewer with display of resolution circles

2013-05-23 Thread Sebastiano Pasqualato
Phenix has a diffraction viewer, too. You may want to try that. Ciao, Sebastiano -- Sebastiano Pasqualato Crystallography Unit Department of Experimental Oncology European Institute of Oncology via Adamello, 16 Milan, Italy On 23/mag/2013, at 10:16, Rafal Dolot rdo...@cbmm.lodz.pl wrote

Re: [ccp4bb] Strand distorsion and residue disconnectivity in pymol

2013-05-30 Thread Sebastiano Pasqualato
Tim, if I'm not wrong, if you judo type dss on the pymol command line you will apply dssp to your pdb and have your model checked for secondary structure elements. Ciao, S -- Sebastiano On 30/mag/2013, at 18:35, Tim Gruene t...@shelx.uni-ac.gwdg.de wrote: -BEGIN PGP SIGNED MESSAGE-

Re: [ccp4bb] Glutathione rsin

2013-06-05 Thread Sebastiano Pasqualato
decent binding capacity? Can they be efficiently regenerated or are they a single use only? Thanks for your help, Mirek -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano

Re: [ccp4bb] announcement: (another) GUI for XDS

2013-06-20 Thread Sebastiano Pasqualato
://strucbio.biologie.uni-konstanz.de email: kay.diederi...@uni-konstanz.de Tel +49 7531 88 4049 Fax 3183 Fachbereich Biologie, Universität Konstanz, Box 647, D-78457 Konstanz -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO

Re: [ccp4bb] announcement: (another) GUI for XDS

2013-06-20 Thread Sebastiano Pasqualato
, Sebastiano On Jun 20, 2013, at 11:18 AM, Kay Diederichs kay.diederi...@uni-konstanz.de wrote: On 06/20/2013 11:07 AM, Sebastiano Pasqualato wrote: Hi Kay, hi all, sorry for the -maybe- naive questions, but i'm struggling to get the GUI for XDS going. I'm on Mac OSX 10.8.4. The dmg

Re: [ccp4bb] announcement: (another) GUI for XDS

2013-06-26 Thread Sebastiano Pasqualato
http://strucbio.biologie.uni-konstanz.de email: kay.diederi...@uni-konstanz.de Tel +49 7531 88 4049 Fax 3183 Fachbereich Biologie, Universität Konstanz, Box 647, D-78457 Konstanz -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European

Re: [ccp4bb] is there any version of xia2 or XDSME compatible with new XDS?

2013-07-03 Thread Sebastiano Pasqualato
wrote: Hi, I found the current release of xia2 or XDSME is not compatible with latest XDS. Is there any similar software available? Thanks! -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16

[ccp4bb] [ot] looking for Crystal Farm 400 service maintenance

2013-07-16 Thread Sebastiano Pasqualato
appreciated, best regards, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990

Re: [ccp4bb] PDB structure validation

2013-10-31 Thread Sebastiano Pasqualato
would improve the quality of deposited structures. Debasish Chattopadhyay University of Alabama at Birmingham CBSE-250 1025 18th Street South, Birmingham, Al-35294 USA Ph: (205)934-0124; Fax: (205)934-0480 -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental

Re: [ccp4bb] Tenure track junior Group Leader Positions in Milan, Italy

2014-04-04 Thread Sebastiano Pasqualato
interesting: “experimental and computational dance biology”. Any type of computational dance or there are style limitations? Regards, Vaheh Oganesyan www.medimmune.com image001.png From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Sebastiano Pasqualato Sent

[ccp4bb] selective protection of C-terminal carboxylate

2012-05-25 Thread Sebastiano Pasqualato
Dear all, is anyone aware of a way to selectively protect the carboxylate at a protein C-terminus, while leaving unaffected those of Asp and Glu side chains? Thanks a lot in advance, have a nice weekend, ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental

[ccp4bb] advice on spectrometric instruments choice

2012-07-20 Thread Sebastiano Pasqualato
the Chirascan from Applied Photophysics, or the Jasco J-815, so far. Any comment on those? Thanks a lot in advance, best regards, ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano

[ccp4bb] modeling thioester bond

2012-07-25 Thread Sebastiano Pasqualato
and it has to be taken into account? Thanks in advance, ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 please note

Re: [ccp4bb] modeling thioester bond

2012-07-26 Thread Sebastiano Pasqualato
Govindpura, Bhopal 462023 India tel: +91-750-4092340 From: Sebastiano Pasqualato sebastiano.pasqual...@gmail.com To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, July 25, 2012 9:48 PM Subject: [ccp4bb] modeling thioester bond No answers yet from

Re: [ccp4bb] Space group R32 and H32

2012-07-30 Thread Sebastiano Pasqualato
because both are used in literature? Thank you. -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 please note the change

Re: [ccp4bb] Space group R32 and H32

2012-07-30 Thread Sebastiano Pasqualato
it 'R32:h' is also completely unambiguous and acceptable (but calling it 'R32' most definitely is not!). For the PDB CRYST1 record you have (unfortunately) to call it 'H32'. Hope this clears it up. -- Ian On 30 July 2012 17:20, Sebastiano Pasqualato sebastiano.pasqual...@gmail.com wrote

Re: [ccp4bb] vitrification vs freezing

2012-11-16 Thread Sebastiano Pasqualato
- http://enigmail.mozdev.org/ iD8DBQFQpg1XUxlJ7aRr7hoRAl33AKCbSYXQmD2YyVug5s3i+2CYDVDzqQCfZ7Qz 4IiEP5B5NrB+D0s+r/tIa6o= =nN9O -END PGP SIGNATURE- -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via

Re: [ccp4bb] vitrification vs freezing

2012-11-16 Thread Sebastiano Pasqualato
learnt a few interesting things. Thank you all for that. It's maybe time to step forward from this thread. Thank you all, once again, ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139

[ccp4bb] changing resolution bins statistics in CORRECT.LP

2013-01-15 Thread Sebastiano Pasqualato
the resolution bins with the keyword RESOLUTION_SHELLS=, but rather the table output by the CORRECT job of XDS. Thanks in advance, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16

Re: [ccp4bb] changing resolution bins statistics in CORRECT.LP

2013-01-16 Thread Sebastiano Pasqualato
the XSCALE statistics instead? The statistics are probably more meaningful after scaling, I guess. Best, Tim On 01/15/2013 04:22 PM, Sebastiano Pasqualato wrote: Hi all, I was wondering if XDS allows to change the number of resolution bins appearing in the table: SUBSET OF INTENSITY DATA

Re: [ccp4bb] changing resolution bins statistics in CORRECT.LP

2013-01-16 Thread Sebastiano Pasqualato
(+), F(-) but also merged F. I was expecting those were merged identically to the case in which FRIEDEL'S_LAW is off. But maybe your point is correct. Thanks, s Cheers, Tim On 01/16/2013 02:03 PM, Sebastiano Pasqualato wrote: Thanks to Kay and Tim or the feedback. The reason I wanted

Re: [ccp4bb] changing resolution bins statistics in CORRECT.LP

2013-01-16 Thread Sebastiano Pasqualato
varieties of FRIEDEL'S_LAW differ in their rejections! HTH, Kay Sebastiano Pasqualato sebastiano.pasqual...@gmail.com schrieb: Thanks to Kay and Tim or the feedback. The reason I wanted to get statistics from the CORRECT step of XDS is that I have refined a structure using the mtz

[ccp4bb] statistics from a structure factors file

2013-01-17 Thread Sebastiano Pasqualato
he used to solve a structure, how can I determine the overall quality of the collected data, without having access to the processing logs? Thanks in advance, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology

Re: [ccp4bb] statistics from a structure factors file

2013-01-17 Thread Sebastiano Pasqualato
January 2013 09:18, Sebastiano Pasqualato sebastiano.pasqual...@gmail.com wrote: Hi all, maybe a silly question, but I can't figure this out. Is there a piece of software to calculate Table I statistics such as Rsym, Mn(I/sigI), Multiplicity, Completeness, from a structure factors file

[ccp4bb] PDB crawler

2013-03-20 Thread Sebastiano Pasqualato
Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990

[ccp4bb] SUMMARY: PDB crawler

2013-03-20 Thread Sebastiano Pasqualato
Fax: 972-8-647-2992 or 972-8-646-1710 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Sebastiano Pasqualato [sebastiano.pasqual...@gmail.com] Sent: Wednesday, March 20, 2013 2:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb

[ccp4bb] MAD/SAD data collection strategy

2007-02-15 Thread Sebastiano Pasqualato
... Let the gurus talk! Any advice is obviously highly appreciated! Thanks in advance, Sebastiano and Claudio -- Sebastiano Pasqualato, PhD IFOM Istituto FIRC di Oncologia Molecolare via Adamello, 16 20139 Milano Italy tel +39 02 574 303 325 fax +39 02 574 303 310

[ccp4bb] HKL2000 with more that 2 million observations

2007-02-21 Thread Sebastiano Pasqualato
-- Sebastiano Pasqualato, PhD IFOM Istituto FIRC di Oncologia Molecolare via Adamello, 16 20139 Milano Italy tel +39 02 574 303 325 fax +39 02 574 303 310

Re: [ccp4bb] heavy metal labelled GTP derivatives

2007-05-07 Thread Sebastiano Pasqualato
Hi Stephen, you can get Iodinated or Brominated nucleotide derivatives from jena bioscience (http://www.jenabioscience.com/cms/en/1/browse/106_nucleotides_and_their_analogs.html) hth, Sebastiano At 02:44 PM 5/7/2007 +0200, Stephen Cusack wrote: Does anyone know of any heavy metal labelled GTP

[ccp4bb] automatic hydrophobic core (buried residues) determination

2007-06-14 Thread Sebastiano Pasqualato
Hi all, does anyone know if there's a (ccp4 or not) program able to list the hydrophobic core residues of a structure? a quick google for it didn't provide any useful hint... thanks in advance, ciao s -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale

[ccp4bb] pymol movies scripts

2007-09-18 Thread Sebastiano Pasqualato
pages that would explain me how to do that? Thanks a lot in advance, best, Sebastiano -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 Milano Italy tel +39 02 9437 5094 fax +39 02 574 303 310 No virus

Re: [ccp4bb] pointless and HKL2000

2008-10-06 Thread Sebastiano Pasqualato
, 2008, 5:04:05 PM, Yue Li wrote: Hi everyone, I would like to us pointless in CCP4 to check the laue group of a crystal. Since pointless needs unmerged data, my question is how to generate the unmerged data for pointless from HKL2000 ? Thanks, Simon -- Sebastiano Pasqualato

[ccp4bb] summary: tricoordinate ion?

2008-11-05 Thread Sebastiano Pasqualato
sent what Wincoot wrote out! ;-)). Next time I'll be surely posting the image to some website. Thanks again, all the best, S -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094

Re: [ccp4bb] OT: VectorNTI alternatives

2009-01-28 Thread Sebastiano Pasqualato
to .gb format. I don't know if a locked up (expired) version permits this and you will have no notice that it is about to expire. -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02

Re: [ccp4bb] Alignment software

2010-05-25 Thread Sebastiano Pasqualato
Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406 -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094 fax +39 02 9437 5990

Re: [ccp4bb] Alignment software

2010-05-26 Thread Sebastiano Pasqualato
, Roger On 25 May 2010 17:50, Sebastiano Pasqualato sebastiano.pasqual...@ifom-ieo-campus.it wrote: Hi there, I remember a friend of mine showing me Aline, and it looked very nice. However, I can't make it work on my MacBook Pro (Mac OS X 10.6.3). With respect to the instruction I found

Re: [ccp4bb] difficult P1 crystal

2010-09-30 Thread Sebastiano Pasqualato
(using balbes, molrep, phaser and epmr). Any suggestion is welcome. Thank you, Mario Milani -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094 fax +39 02 9437 5990

[ccp4bb] relationship between B factors and Koff

2010-11-19 Thread Sebastiano Pasqualato
-- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094 fax +39 02 9437 5990

[ccp4bb] hydrohyapatite column

2010-11-29 Thread Sebastiano Pasqualato
for such columns. Thanks in advance, best, s -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094 fax +39 02 9437 5990

[ccp4bb] [OT] which column to use in SLS/MALS instruments

2011-03-08 Thread Sebastiano Pasqualato
are you guys using in your instruments? Thanks a lot in advance for the feedback, best, ciao Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit IFOM-IEO Campus Cogentech - Consortium for Genomic Technologies via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5172 fax +39 02 9437

[ccp4bb] SUMMARY -until now- [OT] which column to use in SLS/MALS instruments

2011-03-09 Thread Sebastiano Pasqualato
accurate results for both a ~250kDa hexameric enzyme as also for a 0.3 mg/ml injection of an insulin sample. Eventually I think depends on the applications you prefer to have. cheers Nikos Engin Özkan wrote: On 3/8/11 5:03 AM, Sebastiano Pasqualato wrote: On the other hand, GE Healthcare

[ccp4bb] ccp4 on Mac Intel

2011-05-03 Thread Sebastiano Pasqualato
. How do I source this ccp4 installation? Thanks a lot in advance, ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit IFOM-IEO Campus Cogentech - Consortium for Genomic Technologies via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5172 fax +39 02 9437 5990

Re: [ccp4bb] ccp4 on Mac Intel

2011-05-03 Thread Sebastiano Pasqualato
-(0)-121-414 5925 Edgbaston E: k.futte...@bham.ac.uk Birmingham, B15 2TT, UK W: http://tinyurl.com/futterer-lab === On 3 May 2011, at 09:30, Sebastiano Pasqualato wrote: Dear all

Re: [ccp4bb] ccp4 on Mac Intel

2011-05-03 Thread Sebastiano Pasqualato
. All the paths should then be set. Charles Ballard CCP4 On 3 May 2011, at 09:30, Sebastiano Pasqualato wrote: Dear all, I have installed ccp4 with the dmg package, and everything is installed nicely in the Application folder, and I can get ccp4i working just by launching from

[ccp4bb] Technician Position available in Milan

2011-05-12 Thread Sebastiano Pasqualato
attitude to relate with people and work in a team. To apply, please send your CV, together with the name of at least one referee. Informal inquiries are welcome. cheers, Sebastiano PS: sorry for the double posting to those subscribed to both mailing lists. -- Sebastiano Pasqualato, PhD

Re: [ccp4bb] hkl2map on Mac OSX 10.6

2011-07-15 Thread Sebastiano Pasqualato
guess that's not a big deal) Thanks again, ciao, s On Jul 15, 2011, at 11:44 AM, Joachim Reichelt wrote: Just a hint: Subshels do not always use aliases, so put shelx etc. in your path e.g. in /usr/local/bin Am 15.07.11 11:40, schrieb Sebastiano Pasqualato: Hi all, has anybody managed

Re: [ccp4bb] Linux vs MacOS for crystallographic software

2011-09-29 Thread Sebastiano Pasqualato
On Sep 29, 2011, at 2:48 AM, Nat Echols wrote: I don't know of any macromolecular crystallography programs that don't run on Mac - Hey there, does this mean that SHARP works on a Mac? ciao, s -- Sebastiano Pasqualato, PhD Crystallography Unit IFOM-IEO Campus Cogentech - Consortium

Re: [ccp4bb] Linux vs MacOS for crystallographic software

2011-09-29 Thread Sebastiano Pasqualato
Thanks Dirk, that's good news. I'll take a look at it, then. thanks, ciao, s On Sep 29, 2011, at 9:51 AM, Dirk Kostrewa wrote: Yes, SHARP and BUSTER both work on a Mac. Cheers, Dirk. Am 29.09.11 09:45, schrieb Sebastiano Pasqualato: On Sep 29, 2011, at 2:48 AM, Nat Echols wrote

Re: [ccp4bb] Crystallization robot and trypsin

2012-01-24 Thread Sebastiano Pasqualato
Smith http://douglas.co.uk or http://www.douglasinstruments.com Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 hbotti.vcf -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano

Re: [ccp4bb] off-topic: special format for multiple sequence (protein) alignment

2012-02-03 Thread Sebastiano Pasqualato
some of you have seen some programs can do this? Thank you. King regards, Wenhe alignment.png -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5172 fax

[ccp4bb] GST-fusion protein production in insect cells

2012-03-16 Thread Sebastiano Pasqualato
, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990

Re: [ccp4bb] GST-fusion protein production in insect cells

2012-03-16 Thread Sebastiano Pasqualato
, MA 02111 Sebastiano Pasqualato wrote: Dear CCP4ers, Dr. Berger, we have an accumulating series of GST-fusion proteins here that are all displaying the same behavior when expressed in Hi5 insect cells with the MultiBac system. What we are experiencing is a massive production of free

[ccp4bb] error in ccp4i

2009-05-16 Thread Sebastiano Pasqualato
need a software, that's when it goes down!) -- Sebastiano Pasqualato, PhD IFOM-IEO Campus Dipartimento di Oncologia Sperimentale Istituto Europeo di Oncologia via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5094

[ccp4bb] Post-doctoral position, IFOM-IEO-CAMPUS, Milan

2009-10-28 Thread Sebastiano Pasqualato
structure. Conservatively, we feel that this can be certainly achieved within two years Salary. At the IFOM-IEO Campus, post-doctoral salaries ranges within 24000-36000€/yr. Further information as to fringe benefits etc can be found at the Campus web Site. -- Sebastiano Pasqualato, PhD IFOM-IEO

[ccp4bb] [ot]: nedit on Mac 10.10 yosemite

2014-10-29 Thread Sebastiano Pasqualato
Reason: image not found Trace/BPT trap: 5 Seba@host041:~ Anybody knows if there is a fix for that? Thanks in advance, S -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel

[ccp4bb] [OT]: Akta system fill port INV-907 alternative

2015-05-15 Thread Sebastiano Pasqualato
, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 web http://is.gd/IEO_XtalUnit http://is.gd/IEO_XtalUnit

[ccp4bb] crystal imaging system

2017-06-30 Thread Sebastiano Pasqualato
) on those instruments; - information on other instruments you would suggest. Thank you very much in advance, wishing you a pleasant weekend, best regards, ciao, S -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus

[ccp4bb] SUMMARY: looking for paper describing optimisation of crystals using a screen kit as additive

2017-05-09 Thread Sebastiano Pasqualato
ups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf> Thanks everyone for the feedback and help! May the crystals grow happily and diffract nicely, ciao, Sebastiano > On 8 May 2017, at 15:18, Sebastiano Pasqualato > <sebastiano.pasqual...@gmail.com> wrote: > >

[ccp4bb] Electronic Laboratory Notebook

2017-05-19 Thread Sebastiano Pasqualato
a lot, ciao Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 web http://is.gd/IEOXtalUnit

[ccp4bb] preparing DL-Malic acid stock solution

2017-06-08 Thread Sebastiano Pasqualato
a clear solution? Has anyone gone through the same? Thanks in advance, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02

Re: [ccp4bb] preparing DL-Malic acid stock solution

2017-06-09 Thread Sebastiano Pasqualato
so need to gently heat it. >> >> Diana >> >> ** >> Diana R. Tomchick >> Professor >> Departments of Biophysics and Biochemistry >> University of Texas Southwestern Medical Center >> 5323 Harry Hines Blv

[ccp4bb] looking for paper describing optimisation of crystals using a screen kit as additive

2017-05-08 Thread Sebastiano Pasqualato
condition with 25% of a commercial sparse matrix screen kit with the protein in the drop. I can’t find the reference, can anybody help me? Thanks a lot, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO

[ccp4bb] info on BLI instrumentation

2017-05-19 Thread Sebastiano Pasqualato
perform; - easy of use; - cost of performing experiments; - biological/biochemical problems that can addressed will be highly appreciated! thanks a lot in advance, best, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute

[ccp4bb] maltodextrins on superdex columns?

2017-09-04 Thread Sebastiano Pasqualato
dextrans on superdex columns, but their elution buffer is supplemented with 5% ethanol. Is that added to avoid specific binding of the sugars to the column matrix? Is that necessary or can it be excluded? Thanks a lot in advance for any hint, ciao, Sebastiano -- Sebastiano Pasqualato, PhD

Re: [ccp4bb] Introducing Dials User Interface for CCP4

2018-05-07 Thread Sebastiano Pasqualato
Hi Luis, I’m trying to run the Dials user Interface on a Mac (OSX version 10.11.6, Quartz 2.7.11). i go into the directory where the images are, launch the dui and the graphical user interface appears, without any image displayed. When I click on the “select images” button, the program crashes

Re: [ccp4bb] Introducing Dials User Interface for CCP4

2018-05-07 Thread Sebastiano Pasqualato
& Structural Biology > www.xtal.iqfr.csic.es<http://www.xtal.iqfr.csic.es/> > www.xtal.iqfr.csic.es/Cristalografia/<http://www.xtal.iqfr.csic.es/Cristalografia/> > Telf.: +34 917459550 > Consejo Superior de Investigaciones Científicas > Spanish National Research Council

[ccp4bb] low resolution refinement

2018-05-24 Thread Sebastiano Pasqualato
Hi all, just be sure: does Low Resolution Refinement Pipeline in ccp4 always use Free_R_flag=0? Thanks, s -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02

Re: [ccp4bb] size exclusion columns

2018-04-26 Thread Sebastiano Pasqualato
olumn' tips or recommendations when dealing > with large proteins (MDa)? > > Many thanks > Markus -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990

[ccp4bb] CHAPS provider

2018-11-30 Thread Sebastiano Pasqualato
is worth the cost. Could anybody share their experience with low-cost chemical providers, please? Thank you very much, have a nice weekend, Sebastiano -- Sebastiano Pasqualato, PhD Biochemistry and Structural Biology Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus

[ccp4bb] Yeast microtubule model

2019-10-24 Thread Sebastiano Pasqualato
tubulin dimers, but I don’t know exactly how. If someone has already done that and is willing to share it, that would be extremely helpful. Thanks a lot in advance, ciao, S PS: sorry for the cross posting to members of both bulletin boards. -- Sebastiano Pasqualato, PhD Biochemistry

[ccp4bb] Senior Technician Position at the Biochemistry and Structural Biology Unit - IEO Milano

2021-01-11 Thread Sebastiano Pasqualato
nal Electron Microscopy access, which the candidate is expected to manage. Experience in setting up grids and Cryo-EM data collection will be a distinguishing skill. Potential applicants are encouraged to contact Dr. Sebastiano Pasqualato, sending a brief description of interests, and a CV with

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

2021-09-16 Thread Sebastiano Pasqualato
Hi Claude, Pymol 2.4.0 (installed via homebrew) works fine for me in Apple M1 MacBook Pro, 2020. Ciao, S > On 16 Sep 2021, at 12:12, Claude Sauter wrote: > > Dear all, > > to continue on the same theme, a colleague biochemist asked me a couple of > days ago if there is any incompatibility

[ccp4bb] Post doc positions *CryoEM - Vannin Lab* in the newly established Structural Biology Centre of Human Technopole, MIlan

2021-10-04 Thread Sebastiano Pasqualato
The Human Technopole (HT) Foundation is looking for Post-doctoral fellows to work in the Structural Biology Research Centre in the laboratory of Prof. Alessandro Vannini (https://humantechnopole.it/en/research-groups/vannini-group/

[ccp4bb] Staff Scientist opportunity in "Structural Proteomics" at Human Technopole in Milan, Italy

2022-01-14 Thread Sebastiano Pasqualato
o-manage-a-structural-proteomics-facility> Please, share this ad to anyone you think might be interested, ciao, Sebastiano PS: sorry for the double posting in different bulletin boards Sebastiano Pasqualato Bio

[ccp4bb] Staff Scientist opportunity in "Single Molecule Biophysics" at Human Technopole in Milan, Italy

2022-01-20 Thread Sebastiano Pasqualato
st-single-molecule-biophysics Please, share this ad to anyone you think might be interested, ciao, Sebastiano PS: sorry for the double posting in different bulletin boards Sebastiano Pasqualato Biophysic

Re: [ccp4bb] Reg. ITC machine

2023-08-21 Thread Sebastiano Pasqualato
Hello Sivaraman, I completely second Matthew’s message, having had the same experience he had, in two different workplaces and with two different Malvern PEAQ-ITC instruments. I have one anecdotal, and not direct, unpleasant feedback from TA instrument users. Ciao, S > On 9 Aug 2023, at 18:05,

[ccp4bb] NOW HIRING: Structural Proteomics position at Human Technopole, Milano

2024-01-16 Thread Sebastiano Pasqualato
On behalf of Andrea Graziadei: Dear all, the structural proteomics unit of the National Facility for Structural Biology of Human Technopole is looking for a technician! If you’re interested in proteomics, mass spectrometry and structural (systems) biology, please consider applying! The

[ccp4bb] JOB OPPORTUNITY: Technician/Senior Technician in Biophysics in Milan

2024-04-09 Thread Sebastiano Pasqualato
Dear all, please advertise this job opportunity. The Biophysics Unit of the National Facility for Structural Biology of Human Technopole, in Milano, Italy, is seeking to recruit a research technician who will actively participate in maintaining instruments and workflows, take part in