Re: [ccp4bb] AW: [ccp4bb] high Rfree

2017-07-21 Thread Daniel Rigden
Hi there AMPLE works very well for coiled-coil structures, irrespective of parallel/anti-parallel etc http://journals.iucr.org/m/issues/2015/02/00/lz5005/ Resolution permitting, SHELXE tracing and phase modification, built into the AMPLE pipeline, gives statistics that differentiate very

[ccp4bb] AW: [ccp4bb] high Rfree

2017-07-21 Thread Herman . Schreuder
Hi ???, Coiled coil structures can be very tricky with MR, so your solution may not be correct. You could try to split your search model and run MR with the separate coils. If you have high enough resolution (> ~2.3 Å?) and your MR solution is basically correct, you may be able to solve your