Hello, You need to be in the directory above annotationData.
- Dario. ---- Original message ---- >Date: Mon, 27 Sep 2010 16:53:06 -0700 (PDT) >From: aroma-affymetrix@googlegroups.com (on behalf of Wero ><ivansk...@gmail.com>) >Subject: [aroma.affymetrix] Problem with read CDF files >To: "aroma.affymetrix" <aroma-affymetrix@googlegroups.com> >Cc: ii...@inmegen.gob.mx > >Hi, I have been trying to read the CDF files for the HuGene-1_0-st-v1 >array with aroma.affymetrix and it has been very confuse for me. > >I have the correct CDF files from the aroma page. > >The CDF files are in the path: "/Library/Frameworks/R.framework/ >Versions/2.11/Resources/library/aroma.affymetrix/annotationData/ >chipTypes/HuGene-1_0-st-v1". > >My working directory is: annotationData > >but I still have this error: >##################### >> cdf <- AffymetrixCdfFile$byChipType(chipType, tags="r3") >Error in list(`AffymetrixCdfFile$byChipType(chipType, tags = "r3")` = ><environment>, : > >[2010-09-27 06:14:53] Exception: Could not locate a file for this chip >type: HuGene-1_0-st-v1,r3 > at throw(Exception(...)) > at throw.default("Could not locate a file for this chip type: ", pa > at throw("Could not locate a file for this chip type: ", paste(c(ch > at method(static, ...) > at AffymetrixCdfFile$byChipType(chipType, tags = "r3") >################## > >Also, if I look for the cdf path file in R, it returns NULL > >>pathname <- >>"annotationData/chipTypes/HuGene-1_0-st-v1/HuGene-1_0-st-v1,r3.cdf" > >> cdf <- AffymetrixCdfFile(pathname) >Error in list(`AffymetrixCdfFile(pathname)` = <environment>, >`extend(AromaChipTypeAnnotationFile(...), c("AffymetrixCdfFile", ` = ><environment>, : > >[2010-09-27 06:48:54] Exception: Pathname not found: annotationData/ >chipTypes/HuGene-1_0-st-v1/HuGene-1_0-st-v1,r3.cdf (none of the parent >directories [annotationData/chipTypes/HuGene-1_0-st-v1/] exist; >current directory is '/Library/Frameworks/R.framework/Versions/2.11/ >Resources/library/aroma.affymetrix/annotationData/chipTypes/HuGene-1_0- >st-v1') > at throw(Exception(...)) > at throw.default("Pathname not found: ", pathname, reason) > at throw("Pathname not found: ", pathname, reason) > at method(static, ...) > at Arguments$getReadablePathname(filename, path = path, mustExist = > at GenericDataFile(...) > at extend(GenericDataFile(...), "AromaMicroarrayDataFile") > at AromaMicroarrayDataFile(...) > at extend(AromaMicroarrayDataFile(...), c("AffymetrixFile", uses("A > at AffymetrixFile(...) > at extend(AffymetrixFile(...), "AromaChipTypeAnnotationFile") > at AromaChipTypeAnnotationFile(...) > at extend(AromaChipTypeAnnotationFile(...), c("AffymetrixCdfFile", > at Affymetrix >In addition: Warning messages: >1: In is.na(parent) : > is.na() applied to non-(list or vector) of type 'NULL' >2: In is.na(parent) : > is.na() applied to non-(list or vector) of type 'NULL' >> pathname <- getPathname(cdf) >> print(pathname) >[1] "annotationData/chipTypes/HuGene-1_0-st-v1/HuGene-1_0-st- >v1,r3.cdf" > >> pathname2 <- AffymetrixCdfFile$findByChipType("HuGene-1_0-st-v1", tags="3") >> print(pathname2) >NULL > >####################################### >This is my sessionInfo() >R version 2.11.1 (2010-05-31) >i386-apple-darwin9.8.0 > >locale: >[1] en_US.UTF-8/en_US.UTF-8/C/C/en_US.UTF-8/en_US.UTF-8 > >attached base packages: >[1] stats graphics grDevices utils datasets methods >[7] base > >other attached packages: > [1] oligoClasses_1.10.0 Biobase_2.8.0 > [3] aroma.affymetrix_1.7.0 aroma.apd_0.1.7 > [5] affxparser_1.20.0 R.huge_0.2.0 > [7] aroma.core_1.7.0 aroma.light_1.16.1 > [9] matrixStats_0.2.1 R.rsp_0.4.0 >[11] R.cache_0.3.0 R.filesets_0.9.0 >[13] digest_0.4.2 R.utils_1.5.2 >[15] R.oo_1.7.4 oligo_1.12.2 >[17] R.methodsS3_1.2.1 > >loaded via a namespace (and not attached): >[1] affyio_1.16.0 Biostrings_2.16.5 DBI_0.2-5 >[4] IRanges_1.6.6 preprocessCore_1.10.0 splines_2.11.1 >[7] tools_2.11.1 > >#################################### >This is my traceback() >6: throw.Exception(Exception(...)) >5: throw(Exception(...)) >4: throw.default(msg) >3: throw(msg) >2: method(static, ...) >1: AffymetrixCdfFile$fromChipType("HuGene-1_0-st-v1", tags = "r3") > >############################### >I would appreciate any help, thanks!!! > >Wero. > > >-- >When reporting problems on aroma.affymetrix, make sure 1) to run the latest >version of the package, 2) to report the output of sessionInfo() and >traceback(), and 3) to post a complete code example. > > >You received this message because you are subscribed to the Google Groups >"aroma.affymetrix" group with website http://www.aroma-project.org/. >To post to this group, send email to aroma-affymetrix@googlegroups.com >To unsubscribe and other options, go to http://www.aroma-project.org/forum/ -------------------------------------- Dario Strbenac Research Assistant Cancer Epigenetics Garvan Institute of Medical Research Darlinghurst NSW 2010 Australia -- When reporting problems on aroma.affymetrix, make sure 1) to run the latest version of the package, 2) to report the output of sessionInfo() and traceback(), and 3) to post a complete code example. 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