+----[ Marcos Mancilla <[EMAIL PROTECTED]> (13.Jul.2005 17:29):
|
| Hi,
| 
| I am first time ACT user and I am looking for answer for trivial questions,
| but I could not find them in the mailing list archives. I performed
| comparisons between Brucella genomes and they have been already successfully
| completed!!!. But, I have a doubt about the comparisons with B. melitensis
| 16M files. When I performed the comparison between B. melitensis files (EMBL
| or GenBank) vs B. suis or B. abortus files, it is displayed B. melitensis
| genome "flipped" respect B. suis or B. abortus 

Hola Marcos,

yes, the B. melitensis genome is 'flipped' with respect to
the other two, as there is a big rearrangement near the ori.

[snipped]

| I do not know if the screen (almost all blue between genomes and a double
| triangle like an genomic inversion) displayed can be change by flipping the
| B. melitensis sequence before running the comparison (if you know, please
| let me know!!). Is it possible to perform a comparison changing the genome
| orientation of B. melitensis?

What we have done is to use a flipped B. melitensis genome
for the comparisons.

Saludos,

Fernan

| Thanks,
| 
| Marcos Mancilla
| Ph.D. student
|
+----]

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