Hi, I am first time ACT user and I am looking for answer for trivial questions, but I could not find them in the mailing list archives. I performed comparisons between Brucella genomes and they have been already successfully completed!!!. But, I have a doubt about the comparisons with B. melitensis 16M files. When I performed the comparison between B. melitensis files (EMBL or GenBank) vs B. suis or B. abortus files, it is displayed B. melitensis genome "flipped" respect B. suis or B. abortus (e.g: dnaA is the first gen annotated in B.suis & B. abortus genomes, but BMEI1943, homologous in B. melitensis, is 2Mb downstream). Therefore, when the comparison is running, the following message is displayed: "note: hits from http://www.webact.org:80 /webact/generate?CGISESSID=40d714e7d9f9ef3b89f0b47a51c50f2e&rm=mode 4&SAVE= blast_outl.gz have been flipp..." I do not know if the screen (almost all blue between genomes and a double triangle like an genomic inversion) displayed can be change by flipping the B. melitensis sequence before running the comparison (if you know, please let me know!!). Is it possible to perform a comparison changing the genome orientation of B. melitensis?
Thanks, Marcos Mancilla Ph.D. student _______________________________________________ Artemis-users mailing list [email protected] http://lists.sanger.ac.uk/mailman/listinfo/artemis-users
