Thanks, Jari. The problem happened with Base 1 version. We set up your
lowess as standalone version so that we can run command like: cat input.txt
| lowess > output.txt on Solaris unix. 

In input file, we used header like the following. Can we use the same header
format to run your BASE2 Java version? If not, could you provide a header
sample? 

Thanks,
George


BASEfile
section lowess settings
fit_fraction    0.33
min_x_step      0.1
n_iter  4
%

section assays
count   2
columns id      name
annotationColumns
%
1       temp

section spots
columns position        reporter        assayData
assayFields     intensity1      intensity2
assays  1
%
0       1       1276    1491
1       1       2096    790
2       1       1146    5017

-----Original Message-----
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Jari
Häkkinen
Sent: Thursday, May 03, 2007 8:35 AM
To: BASE ML
Subject: Re: [base] Base Lowess normalization program

Hi,

I assume you are referring to the BASE 2 implementation of lowess. The 
lowess plug-in is a part of the BASE core and is available in source 
form in the source package downloadable from the base web site. You can 
browse the code at 
http://base.thep.lu.se/browser/trunk/src/plugins/core/net/sf/basedb/plugins/
LowessNormalization.java

We have no reported issues with the plug-in but we have not tested 244k 
arrays. It may be out of memory issues, can you check the logs for 
messages related to the problem.


Jari


George Wu wrote:
> Dear All:
> 
> I am using Base Lowess program to normalize my Microarray data. But seems
it
> works with my small dataset input (e.g. 44k-probe records). But it doesn't
> work with my big data input file (e.g. 244K-probe array). 
> 
> So does Lowess have data file size limitation? If so, how can I fix it? By
> the way, I only have the executable? Is there any way I could get source
> code?
> 
> Thank you very much,
> 
> George
> 


-------------------------------------------------------------------------
This SF.net email is sponsored by DB2 Express
Download DB2 Express C - the FREE version of DB2 express and take
control of your XML. No limits. Just data. Click to get it now.
http://sourceforge.net/powerbar/db2/
_______________________________________________
The BASE general discussion mailing list
basedb-users@lists.sourceforge.net
unsubscribe: send a mail with subject "unsubscribe" to
[EMAIL PROTECTED]




-------------------------------------------------------------------------
This SF.net email is sponsored by DB2 Express
Download DB2 Express C - the FREE version of DB2 express and take
control of your XML. No limits. Just data. Click to get it now.
http://sourceforge.net/powerbar/db2/
_______________________________________________
The BASE general discussion mailing list
basedb-users@lists.sourceforge.net
unsubscribe: send a mail with subject "unsubscribe" to
[EMAIL PROTECTED]

Reply via email to