hey.. In SDS-PAGE, stacking gel has larger pore size than the resolving gel,,,,,, if u have done SDS-PAGE, u must have maintained different voltage-current when the sample proteins runs through the stacking gel (~70 V) and resolving gel (~ 150 V) ..... it must be due to the same resason different pH should be maintained
On Nov 8, 11:51 am, "Vijayashree Navali" <[EMAIL PROTECTED]> wrote: > hello frnds, > > can anybody explain me y we hv 2 maintain different pH for > separating gel & stacking gel in SDS PAGE??????????? --~--~---------~--~----~------------~-------~--~----~ Please Request your Friend : Join Biofriend India. We assured quality posting Satish Chauk Biofriend India Manager Pune (India) ========================================= Please visit this link & send your friends too http://www.cbclickbank.com/bioinformatics/company.htm Read message on web - Subject - " No email " & email us Remove from group - Subject - " unsubscribe me " & email us for more information visit http://www.cbclickbank.com/bioinformatics/giudence.htm We assure that your query we would answer within 24 Hrs. post message send email - [email protected] -~----------~----~----~----~------~----~------~--~---
