In stacking gel, 6.8 pH is maintained bcz----These conditions provide an environment for Kohlrausch<http://en.wikipedia.org/wiki/Friedrich_Kohlrausch>reactions, as a result, proteins are concentrated to several fold and a thin starting zone of the order of 19 μm is achieved in a few minutes here all glycine will be as zwitter ions and will give uniform neutral charge to protein...
and in resolving gel ---In this gel, macro molecules separate according to their size. and in pH 8 and more..some of the glycine molecules will be in the negative state..giving partial negative charge to the gel....helps in better resolution of the proteins. On Tue, Nov 11, 2008 at 7:02 PM, rms_2043 <[EMAIL PROTECTED]> wrote: > > hey.. > > In SDS-PAGE, stacking gel has larger pore size than the resolving > gel,,,,,, if u have done SDS-PAGE, u must have maintained different > voltage-current when the sample proteins runs through the stacking gel > (~70 V) and resolving gel (~ 150 V) ..... it must be due to the same > resason different pH should be maintained > > > On Nov 8, 11:51 am, "Vijayashree Navali" > <[EMAIL PROTECTED]> wrote: > > hello frnds, > > > > can anybody explain me y we hv 2 maintain different pH > for > > separating gel & stacking gel in SDS PAGE??????????? > > > > --~--~---------~--~----~------------~-------~--~----~ Please Request your Friend : Join Biofriend India. We assured quality posting Satish Chauk Biofriend India Manager Pune (India) ========================================= Please visit this link & send your friends too http://www.cbclickbank.com/bioinformatics/company.htm Read message on web - Subject - " No email " & email us Remove from group - Subject - " unsubscribe me " & email us for more information visit http://www.cbclickbank.com/bioinformatics/giudence.htm We assure that your query we would answer within 24 Hrs. post message send email - [email protected] -~----------~----~----~----~------~----~------~--~---
