Hai frnds,
I need notes for extracellular enzymes and intracellular enzymes. Please tel
me wat to write?
On Tue, Nov 11, 2008 at 10:32 PM, rms_2043 <[EMAIL PROTECTED]> wrote:
>
> hey..
>
> In SDS-PAGE, stacking gel has larger pore size than the resolving
> gel,,,,,, if u have done SDS-PAGE, u must have maintained different
> voltage-current when the sample proteins runs through the stacking gel
> (~70 V) and resolving gel (~ 150 V) ..... it must be due to the same
> resason different pH should be maintained
>
>
> On Nov 8, 11:51 am, "Vijayashree Navali"
> <[EMAIL PROTECTED]> wrote:
> > hello frnds,
> >
> > can anybody explain me y we hv 2 maintain different pH
> for
> > separating gel & stacking gel in SDS PAGE???????????
>
> >
>
--
With lots of luv
Deepi
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