You can just usethe EM image as a search model.
You generate SFS from the EM map, then search with those. You can coax
Amore to do this, also the old ALMN, and maybe MOLREP or PHASER?
Eleanor
Satheesh Kumar Palani Nathan wrote:
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Dear members,
I am trying to use cryo electron microscope images ( of about 9 A
resolution) to build a search model for molecular replacement trials
(against 2.7 A x-ray diffraction data). As, I don’t have any promising
structural homolog for this protein I need to start the model building
from the scratch.
Has any one tried this approach before? Is there any automated
software to build or align secondary structural elements inside an
E-M. Image? Please share your experience and suggestions in this regard.
Thanks in advance
Satheesh
Dr. P.Satheesh Kumar
Postdoctoral Research Associate
Department of Biochemistry and Biophysics
Texas A&M University, 2128 TAMU
College Station, TX - 77843-2128
USA.
Ph. 1-979-862-7639
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