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Hi this is not a crystallography question. I could able to grow reasonable big(0.2-0.3mm) crystals of my protein. I am using 2-2.5% Peg4k/peg 5K(MME),Mg 5 mM, against 1M MgCl2 in reservoir(prot conc. approx. 20mg/ml, in hepes ,7.0,100mM NaCl, 1mMDTT, 10% glycerol). The crystals grow very fast(with in 1 day they appear and grow to max. size by 4 days. They grow at room temp, not at 4 deg. The problem is wheneve they are exposed in air, I see lot of cracks on the crystal surface..I am mounting at room temp. this problem is arising for bigger crystals not for 0.1 mm crystals. Crystals are growing on a skinny precipitate which is difficult to handle during mounting. Have any one experienced this kind of situation? do the crystals diffract with cracks on surface? if not how to tackle this problem?? Thanks Sunando
