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Hi Sunando,
I have experienced a similar situation in the past. In my case, the
crystals were sensitive to the PEG concentration. Using
cryoprotectants with PEG concentrations close to that of the drop
prevented the crystals from cracking. To remove the skinny
precipitate from the crystal, I transferred the crystal to a
reservoir solution diluted in half, and used tools (available from
Hampton Research) to carefully separate the precipitate from the
crystal.
I hope this helps.
Wataru Kagawa
Wataru Kagawa, Ph. D.
Postdoctoral Researcher
Protein Research Group
RIKEN (Physical and Chemical Research Institute)
W221, West Research Bldg.
1-7-22 Suehiro-cho, Tsurumi-ku
Yokohama, Japan 230-0045
tel. 045-503-9206
fax. 045-503-9201
On 2005/10/28, at 6:10, Sunando Datta wrote:
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Hi
this is not a crystallography question. I could able to grow
reasonable
big(0.2-0.3mm) crystals of my protein. I am using 2-2.5% Peg4k/peg
5K(MME),Mg 5 mM, against 1M MgCl2 in reservoir(prot conc. approx.
20mg/ml, in hepes
,7.0,100mM NaCl, 1mMDTT, 10% glycerol). The crystals grow very fast
(with in 1 day
they appear and grow to max. size by 4 days. They grow at room
temp, not
at 4 deg.
The problem is wheneve they are exposed in air, I see lot of
cracks on
the crystal surface..I am mounting at room temp. this problem is
arising
for bigger crystals not for 0.1 mm crystals. Crystals are growing on a
skinny precipitate which is difficult to handle during mounting.
Have any one experienced this kind of situation? do the crystals
diffract
with cracks on surface? if not how to tackle this problem??
Thanks
Sunando
