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Dear all, Recently I obtained protein crystal that grew in 60 % MPD. The crystal diffracts very well to 1.5 Ǻ. It seems that an MPD molecule is in the active center of the enzyme, however the atom C2 of the MPD molecule has a B factor of 2 after several cycles of refinement with Refmac. Does anybody have any idea why the there is such a low B factor for this atom?.The electron density for the region corresponding to the atom C2 of the MPD molecule is visible at 5 sigma level in a 2fo-fc map while at 3 sigma level there is not electron density for the rest of the molecule. I will appreciate any ideas or suggestions. Sincerely, Dario
